Canzian F, Ushijima T, Pascale R, Sugimura T, Dragani T A, Nagao M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Mamm Genome. 1995 Apr;6(4):231-5. doi: 10.1007/BF00352406.
We constructed a tentative genealogic tree of 13 inbred rat strains using genetic markers identified by the AP-PCR (Arbitrarily Primed Polymerase Chain Reaction) technique, which consists of PCR amplification under low stringency conditions, with only one oligonucleotide as both forward and reverse primers. We obtained discrete-state genotypes of 264 loci, presumably covering the whole rat genome. Computational analysis of this panel allowed the construction of a genealogic tree representing the relations among the 13 strains. The accordance of the present results with available data on the histories of some of these strains validates the use of the AP-PCR technique for studies on phylogeny.
我们使用任意引物聚合酶链反应（AP-PCR）技术鉴定的遗传标记构建了13个近交系大鼠品系的初步系谱树。该技术由低严格度条件下的PCR扩增组成，仅使用一个寡核苷酸作为正向和反向引物。我们获得了264个位点的离散状态基因型，推测覆盖了整个大鼠基因组。对该数据集的计算分析使得构建出代表这13个品系之间关系的系谱树成为可能。目前的结果与其中一些品系历史的现有数据相符，这证实了AP-PCR技术在系统发育研究中的应用。
