Yarkoni E, Rapp H J
Cancer Res. 1979 Feb;39(2 Pt 1):535-7.
The influence of mineral oil content on the antitumor activity of emulsified Bacillus Calmette-Guérin cell walls (CW) was studied in mice each with an established transplant of a syngeneic fibrosarcoma. Animals received intratumoral injections of CW (0.02 to 0.6 mg/mouse) emulsified in 1 to 9% oil. The number of animals in which tumor regressed completely depended on the concentration of oil in the emulsion. The methanol extraction residue (MER) of Bacillus Calmette-Guérin administered intratumorally in oil in water emulsion was also immunotherapeutically active. Neither CW nor MER was active when given as aqueous suspensions without oil. Guinea pigs with transplanted hepatomas growing in their skin and with tumor cells in the draining lymph node could be cured of malignant disease by a single intralesional injection of CW or MER (1 mg per guinea pig) if these agents were emulsified in oil (3.3%) and water. Aqueous suspensions of CW and MER were not therapeutically active in the guinea pig.
在患有同基因纤维肉瘤移植瘤的小鼠中，研究了矿物油含量对乳化卡介苗细胞壁（CW）抗肿瘤活性的影响。动物接受瘤内注射乳化在1%至9%油中的CW（0.02至0.6毫克/小鼠）。肿瘤完全消退的动物数量取决于乳液中油的浓度。瘤内注射于水包油乳液中的卡介苗甲醇提取物残渣（MER）也具有免疫治疗活性。当以无油的水性悬浮液给药时，CW和MER均无活性。如果将这些制剂乳化在油（3.3%）和水中，皮肤移植有肝癌且引流淋巴结中有肿瘤细胞的豚鼠，通过单次瘤内注射CW或MER（每只豚鼠1毫克）可治愈恶性疾病。CW和MER的水性悬浮液在豚鼠中无治疗活性。
