Tortoli E, Bartoloni A, Burrini C, Mantella A, Simonetti M T
Bacteriological and Virological Laboratory, Careggi Hospital, Firenze, Italy.
Eur J Clin Microbiol Infect Dis. 1995 Mar;14(3):240-3. doi: 10.1007/BF02310365.
High-performance liquid chromatography (HPLC) has been demonstrated to be a suitable technique for determining the species of mycobacteria on the basis of their mycolic acid pattern. Representative HPLC profiles, which are needed for the visual recognition of chromatograms, have been published for the most frequently encountered mycobacterial species. No extensive study has been reported for less common species, and only a few, scattered chromatographic patterns are available in literature. This study evaluates the utility of this technique for the identification of several rare species. Mycobacterium celatum, Mycobacterium genavense and Mycobacterium simiae chromatographic profiles have been verified, and previously unreported profiles of other species investigated. The chromatographic pattern of Mycobacterium malmoense is presented for the first time.
高效液相色谱法（HPLC）已被证明是一种基于分枝杆菌的分枝菌酸模式来确定其种类的合适技术。已针对最常见的分枝杆菌物种发表了用于色谱图视觉识别所需的代表性HPLC图谱。对于不太常见的物种，尚未有广泛的研究报道，并且文献中仅有少数零散的色谱模式。本研究评估了该技术用于鉴定几种罕见物种的实用性。已验证了塞拉分枝杆菌、日内瓦分枝杆菌和猿分枝杆菌的色谱图谱，并对其他所研究物种以前未报道的图谱进行了研究。首次展示了马尔默分枝杆菌的色谱模式。
