Kirschner P, Springer B, Vogel U, Meier A, Wrede A, Kiekenbeck M, Bange F C, Böttger E C
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
J Clin Microbiol. 1993 Nov;31(11):2882-9. doi: 10.1128/jcm.31.11.2882-2889.1993.
Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.
分枝杆菌属的临床分离株通过聚合酶链反应扩增的16S rRNA基因片段的直接序列测定来鉴定。鉴定基于16S rRNA基因内的一个高变区,其中分枝杆菌菌种以物种特异性核苷酸序列为特征。一个包含52种分枝杆菌特征序列的人工比对数据库能够轻松实现快速且准确的鉴定。本研究结果表明,聚合酶链反应介导的直接序列测定可作为临床实验室中鉴定分枝杆菌的一种快速可靠的方法。此外,现在能够迅速识别以前未描述过的菌种。