Parez J J, Fauville-Dufaux M, Dossogne J L, de Hoffmann E, Pouthier F
Clinical Laboratory, Mont-Godinne University Hospital, Yvoir, Belgium.
Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):717-25. doi: 10.1007/BF02276054.
Gas liquid chromatography (GLC) and thin layer chromatography (TLC) analysis of cell wall content was used for identification of mycobacteria isolated in primary cultures. GLC permitted determination of the fatty acid and alcohol profiles of Mycobacterium simiae and Mycobacterium marinum and detection of a peak in Mycobacterium ulcerans formerly described for Mycobacterium malmoense. Using the data obtained to fill some of the gaps in the dichotomic trees of Tisdall et al. and Jantzen et al., GLC analysis allowed full identification of 8 of 22 mycobacterial species after 24 hours. The other 14 species could be divided into four groups on the basis of similar findings on GLC. TLC was used for full identification of three species. The identification results of conventional methods were concordant with those of GLC and TLC in 161 of 169 strains (93%) representing 21 different species. Using primarily chromatography for analysis of cell wall content, and in the case of some species complementary biochemical tests, the identification procedure could be shortened to a maximum of three days after primary culture.
采用气液色谱法（GLC）和薄层色谱法（TLC）分析细胞壁成分，以鉴定原代培养物中分离出的分枝杆菌。气液色谱法可测定猿猴分枝杆菌和海分枝杆菌的脂肪酸和醇类谱，并检测到溃疡分枝杆菌中一个曾被描述为马尔默分枝杆菌的峰。利用所获得的数据填补蒂兹代尔等人和扬岑等人二分树中的一些空白，气液色谱分析在24小时后可完全鉴定出22种分枝杆菌中的8种。根据气液色谱法的类似结果，其他14种可分为四组。薄层色谱法用于完全鉴定三种分枝杆菌。在代表21个不同物种的169株菌株中，161株（93%）的传统方法鉴定结果与气液色谱法和薄层色谱法的结果一致。主要采用色谱法分析细胞壁成分，对于某些物种则辅以生化试验，鉴定过程可在原代培养后最多缩短至三天。
