Thacker W L, Talkington D F
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
J Clin Microbiol. 1995 May;33(5):1212-4. doi: 10.1128/jcm.33.5.1212-1214.1995.
The complement fixation (CF) test is the current reference serologic test for the diagnosis of Mycoplasma pneumoniae infection. However, it is reported to be insensitive and nonspecific, and it is labor intensive. To determine if a faster and more sensitive diagnosis of M. pneumoniae could be obtained, we examined 50 paired serum samples from patients with suspected M. pneumoniae infection by the CF test and two commercial rapid antibody detection kits, the Remel M. pneumoniae immunoglobulin G (IgG)-IgM antibody test system (Remel, Lenexa, Kans.) and the Seradyn Color Vue M. pneumoniae IgG-IgM kit (Seradyn, Indianapolis, Ind.). The Remel test, a 5-min qualitative immunobinding assay, detected antibodies in three patient serum samples with CF titers of 32 and in all but one sample with titers of > or = 64. The Seradyn test, a 40-min qualitative agglutination test, was less sensitive than CF or Remel. The Seradyn test was positive in 68% of cases, compared with 94 and 96% of cases tested by CF or Remel, respectively. Both commercial tests are faster and less technically demanding to perform than is the CF test.
补体结合（CF）试验是目前用于诊断肺炎支原体感染的参考血清学试验。然而，据报道该试验不敏感且缺乏特异性，并且操作繁琐。为了确定是否能更快、更灵敏地诊断肺炎支原体感染，我们采用CF试验以及两种商用快速抗体检测试剂盒（Remel肺炎支原体免疫球蛋白G（IgG）-IgM抗体检测系统（Remel公司，堪萨斯州莱尼克斯）和Seradyn Color Vue肺炎支原体IgG-IgM试剂盒（Seradyn公司，印第安纳州印第安纳波利斯））对50例疑似肺炎支原体感染患者的配对血清样本进行了检测。Remel试验是一种5分钟的定性免疫结合试验，在CF滴度为32的3例患者血清样本中检测到了抗体，在除1例样本外的所有CF滴度≥64的样本中也检测到了抗体。Seradyn试验是一种40分钟的定性凝集试验，其敏感性低于CF试验或Remel试验。Seradyn试验在68%的病例中呈阳性，而CF试验和Remel试验的阳性率分别为94%和96%。这两种商用试验在操作上都比CF试验更快，且对技术的要求更低。
