用于检测外周血中传染性巨细胞病毒的细胞培养-聚合酶链反应技术
Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.
作者信息
Lipson S M, Ashraf A B, Lee S H, Kaplan M H, Shepp D H
机构信息
Jane and Dayton Brown and Dayton T. Brown Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital, Manhasset, New York 11030, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1411-3. doi: 10.1128/jcm.33.5.1411-1413.1995.
Abstract
We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay-indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.
摘要
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