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1
Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.用于检测外周血中传染性巨细胞病毒的细胞培养-聚合酶链反应技术
J Clin Microbiol. 1995 May;33(5):1411-3. doi: 10.1128/jcm.33.5.1411-1413.1995.
2
Evaluation of CMV-vue antigenemia assay for rapid detection of cytomegalovirus in mixed-leukocyte blood fractions.评估CMV-vue抗原血症检测法在混合白细胞血液组分中快速检测巨细胞病毒的能力。
J Clin Microbiol. 1995 Apr;33(4):1014-5. doi: 10.1128/jcm.33.4.1014-1015.1995.
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Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes.巨细胞病毒（CMV）抗原血症检测在快速检测多形核血白细胞中的CMV方面比空斑试验培养更敏感。
J Clin Microbiol. 1992 Nov;30(11):2822-5. doi: 10.1128/jcm.30.11.2822-2825.1992.
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Multicenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia.巨细胞病毒血症检测中，第二代Digene杂交捕获法检测巨细胞病毒DNA分析、pp65抗原血症分析及细胞培养法的多中心比较。
J Clin Microbiol. 1999 Apr;37(4):958-63. doi: 10.1128/JCM.37.4.958-963.1999.
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Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.比较聚合酶链反应（PCR）、pp65抗原血症检测法与定量空斑试验培养法检测实体器官移植受者血液白细胞中巨细胞病毒的情况。
J Clin Microbiol. 1994 Apr;32(4):997-1003. doi: 10.1128/jcm.32.4.997-1003.1994.
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Monitoring of CMV infection: a comparison of PCR from whole blood, plasma-PCR, pp65-antigenemia and virus culture in patients after bone marrow transplantation.巨细胞病毒感染的监测：骨髓移植患者全血PCR、血浆PCR、pp65抗原血症检测及病毒培养的比较
Bone Marrow Transplant. 1996 May;17(5):861-8.
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Evaluation of the Digene Hybrid Capture System for detection and quantitation of human cytomegalovirus viremia in human immunodeficiency virus-infected patients.评估Digene杂交捕获系统用于检测和定量人类免疫缺陷病毒感染患者的人巨细胞病毒血症。
J Clin Microbiol. 1996 Dec;34(12):2959-62. doi: 10.1128/jcm.34.12.2959-2962.1996.
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Evaluation of a commercial PCR kit for diagnosis of cytomegalovirus infection of the central nervous system.用于诊断中枢神经系统巨细胞病毒感染的商用聚合酶链反应试剂盒的评估
J Clin Microbiol. 1998 Nov;36(11):3382-4. doi: 10.1128/JCM.36.11.3382-3384.1998.
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Improved detection of cytomegalovirus viremia in AIDS patients using shell vial and indirect immunoperoxidase methodologies.采用空斑试验和间接免疫过氧化物酶法改进对艾滋病患者巨细胞病毒血症的检测。
J Med Virol. 1992 Sep;38(1):36-43. doi: 10.1002/jmv.1890380109.
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Evaluation of CMV Brite kit for detection of cytomegalovirus pp65 antigenemia in peripheral blood leukocytes by immunofluorescence.通过免疫荧光法评估CMV Brite试剂盒在外周血白细胞中检测巨细胞病毒pp65抗原血症的效果。
J Clin Microbiol. 1996 May;34(5):1337-9. doi: 10.1128/jcm.34.5.1337-1339.1996.

引用本文的文献

1
Evaluation of the PrimeCapture CMV DNA detection plate system for detection of cytomegalovirus in clinical specimens.评估PrimeCapture巨细胞病毒DNA检测板系统在临床标本中检测巨细胞病毒的性能。
J Clin Microbiol. 1999 Aug;37(8):2587-91. doi: 10.1128/JCM.37.8.2587-2591.1999.
2
Multicenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia.巨细胞病毒血症检测中，第二代Digene杂交捕获法检测巨细胞病毒DNA分析、pp65抗原血症分析及细胞培养法的多中心比较。
J Clin Microbiol. 1999 Apr;37(4):958-63. doi: 10.1128/JCM.37.4.958-963.1999.

本文引用的文献

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Detection of cytomegalovirus in blood donors by the polymerase chain reaction.
Transfusion. 1993 Jun;33(6):497-503. doi: 10.1046/j.1537-2995.1993.33693296813.x.
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Use of the cytomegalovirus antigenemia (CMV-Ag) assay for the detection of CMV in the blood of AIDS patients.利用巨细胞病毒血症（CMV-Ag）检测法检测艾滋病患者血液中的巨细胞病毒。
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Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay.聚合酶链反应在多种标本类型诊断巨细胞病毒感染中的应用：与细胞培养和空斑试验的比较
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Detection of cytomegalovirus DNA in sera of liver transplant recipients.肝移植受者血清中巨细胞病毒DNA的检测
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Association of cytomegalovirus infection with post-transplantation cardiac rejection as studied using the polymerase chain reaction.运用聚合酶链反应研究巨细胞病毒感染与移植后心脏排斥反应的关联。
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Comparison of four latex agglutination (LA) and three enzyme-linked immunosorbent assays (ELISA) for the detection of rotavirus in fecal specimens.
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Reactivation and recombination of multiple cytomegalovirus strains from individual organ donors.来自个体器官供体的多种巨细胞病毒毒株的再激活和重组。
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Northern and Southern blots.Northern杂交和Southern杂交。
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The significance of the detection of cytomegalovirus in the bronchoalveolar lavage fluid in AIDS patients with pneumonia.艾滋病合并肺炎患者支气管肺泡灌洗液中巨细胞病毒检测的意义。
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Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia.通过检测病毒血症、抗原血症和DNA血症监测心脏移植受者的人巨细胞病毒感染及更昔洛韦治疗情况。
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用于检测外周血中传染性巨细胞病毒的细胞培养-聚合酶链反应技术

Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.

作者信息

Lipson S M, Ashraf A B, Lee S H, Kaplan M H, Shepp D H

机构信息

Jane and Dayton Brown and Dayton T. Brown Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital, Manhasset, New York 11030, USA.

出版信息

J Clin Microbiol. 1995 May;33(5):1411-3. doi: 10.1128/jcm.33.5.1411-1413.1995.

DOI:10.1128/jcm.33.5.1411-1413.1995
PMID:7615769
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228181/
Abstract

We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay-indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.

摘要

抱歉，这个问题未找到相关结果。