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用于诊断中枢神经系统巨细胞病毒感染的商用聚合酶链反应试剂盒的评估

Evaluation of a commercial PCR kit for diagnosis of cytomegalovirus infection of the central nervous system.

作者信息

Weinberg A, Spiers D, Cai G Y, Long C M, Sun R, Tevere V

机构信息

Pediatric Infectious Diseases, University of Colorado School of Medicine, Denver, Colorado, USA.

出版信息

J Clin Microbiol. 1998 Nov;36(11):3382-4. doi: 10.1128/JCM.36.11.3382-3384.1998.

DOI:10.1128/JCM.36.11.3382-3384.1998
PMID:9774601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105337/
Abstract

We evaluated the AMPLICOR cytomegalovirus (CMV) PCR kit for the diagnosis of neurologic CMV infections on 43 positive and 112 negative archived cerebrospinal fluid specimens originally tested by an in-house PCR method. The AMPLICOR kit showed sensitivity and specificity of 95 and 100%, respectively, versus the home-grown assay, indicating its utility in this clinical setting.

摘要

我们使用AMPLICOR巨细胞病毒（CMV）PCR试剂盒，对43份最初采用内部PCR方法检测的阳性存档脑脊液标本和112份阴性存档脑脊液标本进行检测，以诊断神经系统CMV感染。与自制检测方法相比，AMPLICOR试剂盒的灵敏度和特异性分别为95%和100%，表明其在该临床环境中的实用性。

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