用于诊断中枢神经系统巨细胞病毒感染的商用聚合酶链反应试剂盒的评估
Evaluation of a commercial PCR kit for diagnosis of cytomegalovirus infection of the central nervous system.
作者信息
Weinberg A, Spiers D, Cai G Y, Long C M, Sun R, Tevere V
机构信息
Pediatric Infectious Diseases, University of Colorado School of Medicine, Denver, Colorado, USA.
出版信息
J Clin Microbiol. 1998 Nov;36(11):3382-4. doi: 10.1128/JCM.36.11.3382-3384.1998.
Abstract
We evaluated the AMPLICOR cytomegalovirus (CMV) PCR kit for the diagnosis of neurologic CMV infections on 43 positive and 112 negative archived cerebrospinal fluid specimens originally tested by an in-house PCR method. The AMPLICOR kit showed sensitivity and specificity of 95 and 100%, respectively, versus the home-grown assay, indicating its utility in this clinical setting.
摘要
我们使用AMPLICOR巨细胞病毒（CMV）PCR试剂盒，对43份最初采用内部PCR方法检测的阳性存档脑脊液标本和112份阴性存档脑脊液标本进行检测，以诊断神经系统CMV感染。与自制检测方法相比，AMPLICOR试剂盒的灵敏度和特异性分别为95%和100%，表明其在该临床环境中的实用性。
相似文献
1
Evaluation of a commercial PCR kit for diagnosis of cytomegalovirus infection of the central nervous system.用于诊断中枢神经系统巨细胞病毒感染的商用聚合酶链反应试剂盒的评估
J Clin Microbiol. 1998 Nov;36(11):3382-4. doi: 10.1128/JCM.36.11.3382-3384.1998.
2
New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.用于快速定量检测脑脊液中人巨细胞病毒的新型聚合酶链反应检测法。
J Clin Microbiol. 1996 Feb;34(2):482-3. doi: 10.1128/jcm.34.2.482-483.1996.
3
PCR assay for detecting porcine cytomegalovirus.用于检测猪巨细胞病毒的聚合酶链反应检测法
J Clin Microbiol. 1999 Nov;37(11):3767-8. doi: 10.1128/JCM.37.11.3767-3768.1999.
4
Quantitation of cytomegalovirus DNA by real-time polymerase chain reaction in peripheral blood specimens of patients with solid organ transplants: comparison with end-point PCR and pp65 antigen test.采用实时聚合酶链反应对实体器官移植患者外周血标本中的巨细胞病毒DNA进行定量分析：与终点PCR及pp65抗原检测的比较
J Med Virol. 2006 Jul;78(7):915-22. doi: 10.1002/jmv.20641.
5
Comparison of a duplex quantitative real-time PCR assay and the COBAS Amplicor CMV Monitor test for detection of cytomegalovirus.用于检测巨细胞病毒的双重定量实时聚合酶链反应检测法与COBAS Amplicor CMV监测检测法的比较
J Clin Microbiol. 2004 May;42(5):1909-14. doi: 10.1128/JCM.42.5.1909-1914.2004.
6
Comparison of three nucleic acid amplification assays of cerebrospinal fluid for diagnosis of cytomegalovirus encephalitis.三种用于诊断巨细胞病毒性脑炎的脑脊液核酸扩增检测方法的比较
J Clin Microbiol. 2001 Mar;39(3):1148-51. doi: 10.1128/JCM.39.3.1148-1151.2001.
7
Detection and quantitation of human cytomegalovirus DNA in faeces.粪便中人类巨细胞病毒DNA的检测与定量
J Virol Methods. 2000 Jan;84(1):1-14. doi: 10.1016/s0166-0934(99)00127-5.
8
Rapid detection of active cytomegalovirus infection by in situ polymerase chain reaction on MRC5 cells inoculated with blood specimens.
J Virol Methods. 1994 Aug;49(1):59-66. doi: 10.1016/0166-0934(94)90055-8.
9
Multiplex, quantitative, real-time PCR assay for cytomegalovirus and human DNA.用于巨细胞病毒和人类DNA的多重定量实时聚合酶链反应检测法
J Clin Microbiol. 2002 Jul;40(7):2381-6. doi: 10.1128/JCM.40.7.2381-2386.2002.
10
Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.用于检测外周血中传染性巨细胞病毒的细胞培养-聚合酶链反应技术
J Clin Microbiol. 1995 May;33(5):1411-3. doi: 10.1128/jcm.33.5.1411-1413.1995.
引用本文的文献
1
Clinical utility of viral load in management of cytomegalovirus infection after solid organ transplantation.病毒载量在实体器官移植后巨细胞病毒感染管理中的临床应用。
Clin Microbiol Rev. 2013 Oct;26(4):703-27. doi: 10.1128/CMR.00015-13.
2
Human herpesviruses 6 and 7 and central nervous system infection in children.人类疱疹病毒6型和7型与儿童中枢神经系统感染
Emerg Infect Dis. 2004 Aug;10(8):1450-4. doi: 10.3201/eid1008.030788.
3
Molecular methods for diagnosis of viral encephalitis.病毒性脑炎的分子诊断方法。
Clin Microbiol Rev. 2004 Oct;17(4):903-25, table of contents. doi: 10.1128/CMR.17.4.903-925.2004.
4
Molecular methods for cytomegalovirus surveillance in bone marrow transplant recipients.骨髓移植受者巨细胞病毒监测的分子方法
J Clin Microbiol. 2002 Nov;40(11):4203-6. doi: 10.1128/JCM.40.11.4203-4206.2002.
5
Molecular Techniques in the Diagnosis of Central Nervous System Infections.中枢神经系统感染诊断中的分子技术
Curr Infect Dis Rep. 2002 Aug;4(4):339-350. doi: 10.1007/s11908-002-0027-x.
6
Comparison of three nucleic acid amplification assays of cerebrospinal fluid for diagnosis of cytomegalovirus encephalitis.三种用于诊断巨细胞病毒性脑炎的脑脊液核酸扩增检测方法的比较
J Clin Microbiol. 2001 Mar;39(3):1148-51. doi: 10.1128/JCM.39.3.1148-1151.2001.
7
Comparison of PCR, antigenemia assay, and rapid blood culture for detection and prevention of cytomegalovirus disease after lung transplantation.肺移植后检测和预防巨细胞病毒病的聚合酶链反应、抗原血症检测和快速血培养的比较
J Clin Microbiol. 2000 Feb;38(2):768-72. doi: 10.1128/JCM.38.2.768-772.2000.
8
New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients.实体器官移植受者巨细胞病毒感染及疾病的预防和治疗新策略。
Clin Microbiol Rev. 2000 Jan;13(1):83-121, table of contents. doi: 10.1128/CMR.13.1.83.
9
Evaluation of PCR primers for cytomegalovirus detection.用于巨细胞病毒检测的聚合酶链反应引物评估
J Clin Microbiol. 1999 May;37(5):1658-9. doi: 10.1128/JCM.37.5.1658-1659.1999.
本文引用的文献
1
Risk for retinitis in patients with AIDS can be assessed by quantitation of threshold levels of cytomegalovirus DNA burden in blood.艾滋病患者发生视网膜炎的风险可通过对血液中巨细胞病毒DNA载量阈值水平进行定量分析来评估。
J Infect Dis. 1997 Nov;176(5):1146-55. doi: 10.1086/514106.
2
Diagnosis of cytomegalovirus (CMV) polyradiculopathy and documentation of in vivo anti-CMV activity in cerebrospinal fluid by using branched DNA signal amplification and antigen assays.
J Infect Dis. 1997 Aug;176(2):348-52. doi: 10.1086/514051.
3
Viral meningitis in a preadolescent child caused by reactivation of latent herpes simplex (type 1).
Pediatr Infect Dis J. 1997 Jun;16(6):627-9. doi: 10.1097/00006454-199706000-00017.
4
Cytomegalovirus encephalitis.巨细胞病毒性脑炎
Ann Intern Med. 1996 Oct 1;125(7):577-87. doi: 10.7326/0003-4819-125-7-199610010-00008.
5
AIDS dementia complex complicated by cytomegalovirus encephalopathy.
J Neurol. 1993;240(4):223-31. doi: 10.1007/BF00818709.
6
Detection of cytomegalovirus in cerebrospinal fluid autopsy specimens from AIDS patients.艾滋病患者脑脊液尸检标本中巨细胞病毒的检测
J Infect Dis. 1994 Mar;169(3):623-7. doi: 10.1093/infdis/169.3.623.
7
Cytomegalovirus encephalitis in acquired immunodeficiency syndrome (AIDS).获得性免疫缺陷综合征（艾滋病）中的巨细胞病毒性脑炎。
Neurology. 1994 Mar;44(3 Pt 1):507-14. doi: 10.1212/wnl.44.3_part_1.507.
8
Cytomegalovirus infections of the nervous system in patients with AIDS.
Clin Infect Dis. 1995 Apr;20(4):747-54. doi: 10.1093/clinids/20.4.747.
9
Level of cytomegalovirus (CMV) DNA in cerebrospinal fluid of subjects with AIDS and CMV infection of the central nervous system.患有艾滋病且中枢神经系统感染巨细胞病毒（CMV）的受试者脑脊液中巨细胞病毒（CMV）DNA的水平。
J Infect Dis. 1995 Aug;172(2):527-31. doi: 10.1093/infdis/172.2.527.
10
Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders.
J Infect Dis. 1992 Dec;166(6):1416-21. doi: 10.1093/infdis/166.6.1416.
Zotero 插件