Berstein L M, Pravosudov I V, Kryukova O G
N.N. Petrov Research Institute of Oncology, St. Petersburg, Russia.
Neoplasma. 1995;42(2):57-61.
The purpose of this study was to investigate the peculiarities of hormonal regulation of adenylate cyclase (AC) of blood lymphocytes in colorectal cancer patients and to compare these peculiarities with hormone sensitivity of AC of colorectal tumors and normal colonic mucosa. Basal and stimulated lymphocyte AC activity was studied in 51 healthy persons and 52 cancer patients (14 with colon cancer, 21 with rectal cancer and 17 with stomach cancer) aged 20-75 years. In 31 of 35 patients with colorectal cancer the AC activity was studied simultaneously in lymphocytes, tumor tissue and normal colonic mucosa. To evaluate basal and stimulated AC activity the measurement of c-AMP (Amersham kits) formed in the presence of ATP regenerating system was used. Basal and by VIP, pentagastrin and sodium fluoride stimulated AC activity in lymphocytes of gastrointestinal cancer patients was lower than in lymphocytes of healthy subjects of similar age. Stage dependence of the parameters under study was not found. There was a tendency for higher basal and stimulated lymphocyte AC activity in colon cancer patients as compared to stomach and rectal cancer patients. In colorectal cancer patients the peculiarities of lymphocyte AC reactions to stimulation were closer to those in tumor tissue but not to those in normal colonic mucosa. The reaction of lymphocyte AC to VIP and glucagon coincided more frequently with tumor AC reactions to the same hormones in case of hormone nonsensitive tumors. Thus, basal and stimulated lymphocyte AC activity in colorectal cancer patients was modified to some degree by tumor factors. Lymphocyte AC reactions to VIP and glucagon may be considered as indirect markers of hormone sensitivity of colonic tumors. Moreover, the probability of discovery of hormone nonsensitive tumors by this way is more reliable than hormone sensitive ones.
本研究的目的是调查结直肠癌患者血液淋巴细胞腺苷酸环化酶（AC）的激素调节特性，并将这些特性与结直肠肿瘤和正常结肠黏膜AC的激素敏感性进行比较。对51名20 - 75岁的健康人和52名癌症患者（14例结肠癌、21例直肠癌和17例胃癌）的基础和刺激淋巴细胞AC活性进行了研究。在35例结直肠癌患者中的31例，同时对淋巴细胞、肿瘤组织和正常结肠黏膜的AC活性进行了研究。为评估基础和刺激AC活性，采用了在ATP再生系统存在下形成的c - AMP（Amersham试剂盒）的测量方法。胃肠道癌症患者淋巴细胞中基础和由血管活性肠肽（VIP）、五肽胃泌素和氟化钠刺激的AC活性低于同龄健康受试者淋巴细胞中的活性。未发现所研究参数的分期依赖性。与胃癌和直肠癌患者相比，结肠癌患者基础和刺激淋巴细胞AC活性有升高趋势。在结直肠癌患者中，淋巴细胞AC对刺激反应的特性更接近肿瘤组织而非正常结肠黏膜。在激素不敏感肿瘤的情况下，淋巴细胞AC对VIP和胰高血糖素的反应与肿瘤AC对相同激素的反应更常一致。因此，结直肠癌患者基础和刺激淋巴细胞AC活性在一定程度上受到肿瘤因素的影响。淋巴细胞AC对VIP和胰高血糖素的反应可被视为结肠肿瘤激素敏感性的间接标志物。此外，通过这种方式发现激素不敏感肿瘤的可能性比激素敏感肿瘤更可靠。
