Bogan B W, Lamar R T
Department of Cell and Molecular Biology, University of Wisconsin-Madison, USA.
Appl Environ Microbiol. 1995 Jul;61(7):2631-5. doi: 10.1128/aem.61.7.2631-2635.1995.
The abilities of whole cultures of Phanerochaete chrysosporium and P. chrysosporium manganese peroxidase-mediated lipid peroxidation reactions to degrade the polycyclic aromatic hydrocarbons (PAHs) found in creosote were studied. The disappearance of 12 three- to six-ring PAHs occurred in both systems. Both in vivo and in vitro, the disappearance of all PAHs was found to be very strongly correlated with ionization potential. This was true even for compounds beyond the ionization potential thresholds of lignin peroxidase and Mn3+. Deviations from this correlation were seen in the cases of PAHs which are susceptible to radical addition reactions. These results thus begin to clarify the mechanisms of non-lignin peroxidase-labile PAH degradation in the manganese peroxidase-lipid peroxidation system and provide further evidence for the ability of this system to explain the in vivo oxidation of these compounds.
研究了黄孢原毛平革菌的全培养物以及黄孢原毛平革菌锰过氧化物酶介导的脂质过氧化反应降解杂酚油中多环芳烃（PAHs）的能力。在这两个系统中，12种三至六环PAHs均消失。在体内和体外，均发现所有PAHs的消失与电离势密切相关。即使对于超过木质素过氧化物酶和Mn3 +电离势阈值的化合物也是如此。在易发生自由基加成反应的PAHs情况下，观察到与这种相关性的偏差。因此，这些结果开始阐明锰过氧化物酶 - 脂质过氧化系统中不易被木质素过氧化物酶降解的PAH的降解机制，并为该系统解释这些化合物体内氧化的能力提供了进一步的证据。
