Asturias J A, Arilla M C, Gómez-Bayón N, Martínez A, Martínez J, Palacios R
IFIDESA-ARISTEGUI, Research and Development Department, Bilbao, Spain.
Biochim Biophys Acta. 1998 Apr 1;1397(1):27-30. doi: 10.1016/s0167-4781(98)00006-2.
The cDNA encoding an allergen from the dust mite Dermatophagoides pteronyssinus has been cloned and sequenced. The allergen (Der p 10) is a tropomyosin that shared more than 65% identical residues with other invertebrate tropomyosins. The final recovery of recombinant Der p 10 from the culture media after a single purification step was as much as 26 mg/l. The recombinant allergen is reactive to shrimp antitropomyosin IgG antibodies and has a 5.6% frequency of IgE reactivity in sera from mite-allergic patients.
编码来自尘螨（屋尘螨）变应原的互补脱氧核糖核酸（cDNA）已被克隆和测序。该变应原（Der p 10）是一种原肌球蛋白，与其他无脊椎动物原肌球蛋白有超过65%的相同残基。经过单一纯化步骤后，从培养基中最终回收的重组Der p 10高达26毫克/升。该重组变应原与虾抗原肌球蛋白IgG抗体有反应，在螨过敏患者血清中的IgE反应频率为5.6%。
