Sterne J A, Turner A C, Fine P E, Parry J V, Lucas S B, Pönnighaus J M, Mkandwire P K, Nyasulu S, Warndorff D K
Communicable Disease Epidemiology Unit, London School of Hygiene and Tropical Medicine, United Kingdom.
J Infect Dis. 1995 Aug;172(2):543-6. doi: 10.1093/infdis/172.2.543.
During a large epidemiologic study in the Karonga District of northern Malawi, serum samples from 139 patients with incident leprosy, 124 with newly diagnosed leprosy, 277 patients with incident tuberculosis, and 2296 controls were tested for antibodies to human immunodeficiency virus. Sera were tested according to a four-test protocol using two ELISAs and two particle agglutination assays. Overall, 188 samples were considered positive, 2634 were considered negative, and 14 were indeterminate. All 18 available positive specimens from leprosy patients, a random sample of 14 positive specimens from tuberculosis patients, and 15 positive specimens from controls were tested by Western blot. There was no evidence of substantial numbers of ELISA false-positives in any patient group or among controls.
在马拉维北部卡龙加区进行的一项大型流行病学研究中，对139例麻风病新发患者、124例新诊断麻风病患者、277例结核病新发患者的血清样本以及2296名对照者的血清样本进行了人类免疫缺陷病毒抗体检测。血清按照一种四项检测方案进行检测，该方案使用两种酶联免疫吸附测定法（ELISA）和两种颗粒凝集试验。总体而言，188份样本被视为阳性，2634份被视为阴性，14份结果不确定。对所有18份来自麻风病患者的可用阳性标本、从结核病患者中随机抽取的14份阳性标本以及15份来自对照者的阳性标本进行了蛋白质印迹法检测。在任何患者组或对照者中均未发现大量ELISA假阳性的证据。
