聚合酶链反应在临床标本中检测沙眼衣原体的应用及其与市售检测方法的比较。
Use of the polymerase chain reaction for the detection of Chlamydia trachomatis in clinical specimens and its comparison to commercially available tests.
作者信息
Martin J L, Alexander S Y, Selwood T S, Cross G F
机构信息
Department of Microbiology, Monash University Medical School, Prahran, Victoria, Australia.
出版信息
Genitourin Med. 1995 Jun;71(3):169-71. doi: 10.1136/sti.71.3.169.
Abstract
A polymerase chain reaction (PCR) was developed to detect Chlamydia trachomatis in genital tract specimens. Two sets of primers for the PCR were used; one set amplifies a region of the plasmid present in all C trachomatis strains and the other amplifies a conserved region of the genome coding for the major outer membrane protein. The sensitivity of these PCRs were compared with each other, and with the sensitivities of antigen ELISA, Clearview and culture. Southern blotting and probing was used to increase sensitivity of detection.
摘要
开发了一种聚合酶链反应（PCR）来检测生殖道标本中的沙眼衣原体。该PCR使用了两组引物；一组扩增所有沙眼衣原体菌株中存在的质粒区域，另一组扩增编码主要外膜蛋白的基因组保守区域。将这些PCR的灵敏度相互比较，并与抗原ELISA、Clearview和培养法的灵敏度进行比较。使用Southern印迹和探针来提高检测灵敏度。
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