Reid C B, Liang I, Walsh C
Department of Neurology, Beth Israel Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Neuron. 1995 Aug;15(2):299-310. doi: 10.1016/0896-6273(95)90035-7.
Cell lineage analysis in the cortex has revealed two clonal patterns, clustered and widespread clones. To determine the relationship of these patterns, progenitor cells were infected with a retroviral library encoding alkaline phosphatase, and cortical sibling cells were identified using PCR. Clones labeled at E15 consisted of single cells or small cells clusters (52%) or of widespread cells (48%). However, widespread clones consisted of multiple neuronal or glial cell types, spaced systematically at 2-3 mm intervals. The data suggest that migratory multipotential progenitors divide asymmetrically at intervals defined by cell cycle length, producing single cells or clusters of cells in different cortical regions. Transition from multipotentiality to more restricted potential may correspond to changes in migratory behavior.
对皮质中的细胞谱系分析揭示了两种克隆模式，即聚集克隆和广泛分布的克隆。为了确定这些模式之间的关系，用编码碱性磷酸酶的逆转录病毒文库感染祖细胞，并通过聚合酶链反应（PCR）鉴定皮质中的同胞细胞。在胚胎第15天标记的克隆由单个细胞或小细胞簇（52%）或广泛分布的细胞（48%）组成。然而，广泛分布的克隆由多种神经元或神经胶质细胞类型组成，以2-3毫米的间隔有规律地分布。数据表明，迁移性多能祖细胞在由细胞周期长度定义的间隔内进行不对称分裂，在不同的皮质区域产生单个细胞或细胞簇。从多能性向更有限潜能的转变可能与迁移行为的变化相对应。
