Smith D L, Fedoroff N V
Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA.
Plant Cell. 1995 Jun;7(6):735-45. doi: 10.1105/tpc.7.6.735.
We describe a gene that is expressed in lateral and adventitious root primordia of Arabidopsis. The gene was identified by expression of a transposon-borne promoterless beta-glucuronidase gene in lateral root primordia. The gene, designated LRP1 for lateral root primordium 1, and its corresponding cDNA were cloned and sequenced. The expression pattern of the gene in lateral root primordia was confirmed by in situ hybridization with LRP1 cDNA probes. The LRP1 gene encodes a novel protein. LRP1 expression is activated during the early stages of root primordium development and is turned off prior to the emergence of lateral roots from the parent root. Insertion of the transposon in the LRP1 gene disrupted its expression. To evaluate the homozygous insertion line for a mutant phenotype, several aspects of wild-type lateral root development were analyzed. A mutant phenotype has not yet been identified in the insertion line; however, there is evidence that the gene belongs to a small gene family. LRP1 provides a molecular marker to study the early stages of lateral and adventitious root primordium development.
我们描述了一个在拟南芥侧根和不定根原基中表达的基因。该基因是通过转座子携带的无启动子β-葡萄糖醛酸酶基因在侧根原基中的表达而被鉴定出来的。这个被命名为LRP1（侧根原基1）的基因及其相应的cDNA被克隆并测序。通过用LRP1 cDNA探针进行原位杂交，证实了该基因在侧根原基中的表达模式。LRP1基因编码一种新型蛋白质。LRP1的表达在根原基发育的早期阶段被激活，并在侧根从母根中出现之前关闭。转座子插入LRP1基因会破坏其表达。为了评估纯合插入系的突变表型，分析了野生型侧根发育的几个方面。在插入系中尚未鉴定出突变表型；然而，有证据表明该基因属于一个小基因家族。LRP1为研究侧根和不定根原基发育的早期阶段提供了一个分子标记。
