一种类雪山病毒杯状病毒衣壳蛋白的表达、自组装及抗原性
Expression, self-assembly, and antigenicity of a snow mountain agent-like calicivirus capsid protein.
作者信息
Jiang X, Matson D O, Ruiz-Palacios G M, Hu J, Treanor J, Pickering L K
机构信息
Center for Pediatric Research, Children's Hospital of The King's Daughters, Eastern Virginia Medical School, Norfolk 23510-1001, USA.
出版信息
J Clin Microbiol. 1995 Jun;33(6):1452-5. doi: 10.1128/jcm.33.6.1452-1455.1995.
Abstract
Virus-like particles were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of MX virus, a Mexican strain of human calicivirus. These recombinant MX (rMX) particles were morphologically similar to recombinant Norwalk virus (rNV) particles as observed under an electron microscope and contained a single capsid protein with a molecular weight of 57,000, which was slightly smaller than that of rNV. This protein was immunoprecipitated by sera from volunteers infected with the Snow Mountain agent, but it reacted weakly with sera from volunteers infected with NV. This protein did not react with hyperimmune antisera from animals immunized with rNV in the rNV antigen enzyme immunoassay (EIA). Seroresponses were detected from volunteers infected with Snow Mountain agent and Hawaii agent when the rMX particles were used as antigen in an EIA. This EIA also detected an immune response in the sera of child from whom the MX virus was isolated, and a high prevalence of antibody to MX virus was found in the sera of a cohort of Mexican children.
摘要
在感染了含有墨西哥人杯状病毒MX病毒衣壳基因的重组杆状病毒的昆虫细胞中产生了病毒样颗粒。如在电子显微镜下观察到的，这些重组MX（rMX）颗粒在形态上与重组诺如病毒（rNV）颗粒相似，并且包含一种分子量为57,000的单一衣壳蛋白，该蛋白略小于rNV的衣壳蛋白。这种蛋白被感染雪山因子的志愿者血清免疫沉淀，但与感染NV的志愿者血清反应较弱。在rNV抗原酶免疫测定（EIA）中，该蛋白不与用rNV免疫的动物的超免疫抗血清反应。当rMX颗粒用作EIA中的抗原时，检测到感染雪山因子和夏威夷因子的志愿者的血清反应。该EIA还检测到从其分离出MX病毒的儿童血清中的免疫反应，并且在一群墨西哥儿童的血清中发现了针对MX病毒的高抗体流行率。
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