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Determination of biothiols by bromobimane labeling and high-performance liquid chromatography.

作者信息

Newton G L, Fahey R C

机构信息

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093, USA.

出版信息

Methods Enzymol. 1995;251:148-66. doi: 10.1016/0076-6879(95)51118-0.

DOI:10.1016/0076-6879(95)51118-0
PMID:7651194
Abstract
摘要

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Determination of biothiols by bromobimane labeling and high-performance liquid chromatography.通过溴代双硫腙标记和高效液相色谱法测定生物硫醇
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Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography.使用单溴代双硫腙荧光标记和高效液相色谱法测定低分子量硫醇
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para-sulfobenzoyloxybromobimane: a new membrane-impermeable reagent useful for the analysis of thiols and their export from cells.对-磺酰苯甲酰氧基溴代双硫腙:一种新型的膜不通透性试剂,可用于分析硫醇及其从细胞中的输出。
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Methodologies for the application of monobromobimane to the simultaneous analysis of soluble and protein thiol components of biological systems.将一溴代双硫烷应用于生物系统中可溶性和蛋白质硫醇成分同步分析的方法。
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Trypanothione and N1-glutathionylspermidine: isolation and determination.锥虫硫醇和N1-谷胱甘肽亚精胺:分离与测定。
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Assay of thiols and disulfides in intestinal lymph.肠道淋巴中硫醇和二硫化物的测定
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The evolution of glutathione metabolism in phototrophic microorganisms.光合微生物中谷胱甘肽代谢的进化
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Measurements of glutathione and other thiols in cells and tissues: a simplified procedure based on the HPLC separation of monobromobimane derivatives of thiols.细胞和组织中谷胱甘肽及其他硫醇的测定:基于硫醇单溴代联苯胺衍生物高效液相色谱分离的简化方法。
Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1503-6. doi: 10.1016/0360-3016(84)90490-5.

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