用于检测重金属遗传毒性的微生物测试方法比较。
Comparison of microbial tests for the detection of heavy metal genotoxicity.
作者信息
Codina J C, Pérez-Torrente C, Pérez-García A, Cazorla F M, de Vicente A
机构信息
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Spain.
出版信息
Arch Environ Contam Toxicol. 1995 Aug;29(2):260-5. doi: 10.1007/BF00212978.
Heavy metal genotoxicity was evaluated by using different microbial tests. Four genotoxicity assays were employed: the Ames test, the E. coli WP2 test, the Mutatox test detecting mutagenicity, and the SOS assay with E. coli-detecting enzyme induction. All the metals tested (cadmium, chromium, copper, mercury, nickel, and zinc) were detected as genotoxic by the Mutatox and the SOS tests. The Ames test and the E. coli WP2 assay only detected chromium as genotoxic, causing a mutagenic effect. The sensitivity to metals of all the assays used was maintained when they were dissolved in sewage, although there was a slight increase in the sensitivity thresholds.
通过使用不同的微生物试验评估重金属的遗传毒性。采用了四种遗传毒性测定方法：艾姆斯试验、大肠杆菌WP2试验、检测致突变性的Mutatox试验以及检测大肠杆菌酶诱导的SOS试验。Mutatox试验和SOS试验检测出所有测试金属（镉、铬、铜、汞、镍和锌）均具有遗传毒性。艾姆斯试验和大肠杆菌WP2试验仅检测出铬具有遗传毒性，会产生致突变作用。当所有使用的测定方法溶解于污水中时，它们对金属的敏感性得以保持，尽管敏感性阈值略有增加。
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