Haque M, Hirai Y, Yokota K, Oguma K
Department of Bacteriology, Okayama University Medical School, Japan.
J Bacteriol. 1995 Sep;177(18):5334-7. doi: 10.1128/jb.177.18.5334-5337.1995.
The lipids of different species of Helicobacter (H.felis, H. muridarum, H. mustelae, H. fennelliae, and H. cinaedi) were studied. Different types of cholesteryl glucosides were found in all of the species studied except H. cinaedi. The total amount of cholesteryl glucosides varied from 14.8% of total lipids in H. mustelae to 33.1% of total lipids in H. felis. The different types of cholesteryl glucosides and their species distribution are cholesteryl-6-O-acyl-alpha-D-glucopyranoside (cholesteryl-6-O-tetradecanoyl-alpha-D-glucopyranoside in H. felis and cholesteryl-6-O-dodecanoyl-alpha-D-glucopyranoside in H. muridarum), cholesteryl-alpha-D-glucopyranoside (H. felis, H. muridarum, H. mustelae, and H. fennelliae), and cholesteryl-6-O-phosphatidyl-alpha-D-glucopyranoside (H. fennelliae). The neutral lipid fractions showed a high percentage of cholesterol, with selective accumulation of free cholesterol. The study thus shows that the characteristic presence of steryl glycosides in Helicobacter spp. may be an important chemotaxonomic marker for many of the species, and the helicobacters show a selective accumulation of free cholesterol from the media.
对不同种幽门螺杆菌（猫幽门螺杆菌、鼠幽门螺杆菌、鼬幽门螺杆菌、芬内利幽门螺杆菌和西奈幽门螺杆菌）的脂质进行了研究。除西奈幽门螺杆菌外，在所有研究的菌种中均发现了不同类型的胆固醇葡萄糖苷。胆固醇葡萄糖苷的总量在鼬幽门螺杆菌中占总脂质的14.8%，在猫幽门螺杆菌中占总脂质的33.1%。不同类型的胆固醇葡萄糖苷及其菌种分布为胆固醇-6-O-酰基-α-D-吡喃葡萄糖苷（猫幽门螺杆菌中的胆固醇-6-O-十四烷酰基-α-D-吡喃葡萄糖苷和鼠幽门螺杆菌中的胆固醇-6-O-十二烷酰基-α-D-吡喃葡萄糖苷）、胆固醇-α-D-吡喃葡萄糖苷（猫幽门螺杆菌、鼠幽门螺杆菌、鼬幽门螺杆菌和芬内利幽门螺杆菌）以及胆固醇-6-O-磷脂酰-α-D-吡喃葡萄糖苷（芬内利幽门螺杆菌）。中性脂质组分显示胆固醇比例较高，存在游离胆固醇的选择性积累。因此，该研究表明幽门螺杆菌属中甾体糖苷的特征性存在可能是许多菌种的重要化学分类学标志物，并且幽门螺杆菌显示出从培养基中选择性积累游离胆固醇的现象。
