Kimman T G, Bianchi A T, Wensvoort G, de Bruin T G, Meliefste C
Department of Virology, Central Veterinary Institute, Lelystad, The Netherlands.
J Virol. 1993 May;67(5):2922-7. doi: 10.1128/JVI.67.5.2922-2927.1993.
T-cell responses of pigs to hog cholera virus (HCV) have reportedly been absent or difficult to detect. Therefore, little is known about cellular immunity to HCV. In this study, we used an attenuated strain of pseudorabies virus expressing the envelope glycoprotein E1 of HCV and purified recombinant E1 to examine whether the E1 protein is a target antigen recognized by the T cells of HCV-immune pigs. We were unable to identify the E1 protein as a major target antigen recognized by the T cells of HCV-immune animals. However, such cells proliferated in vitro upon stimulation with viable HCV antigen. The lymphoproliferative response to HCV was strictly time and dose dependent and could be induced upon stimulation by live but not by UV light-inactivated HCV. Depletion studies demonstrated that lymphoproliferation depended on the presence of CD2+CD8bright+ lymphocytes, but CD2+CD4+ cells also contributed to the lymphoproliferative response. The primary lymphoproliferative response in animals inoculated with 10(7) 50% tissue culture infective doses of strain Brescia 2.1.1 was stronger than that observed in animals inoculated with 10(3) 50% tissue culture infective doses of the Cedipest strain. A remarkable finding was the increase in non-antigen-specific lymphoproliferation upon inoculation of the animals with HCV strains. This immunological phenomenon may mask a specific T-cell response to the virus.
据报道，猪对猪瘟病毒（HCV）的T细胞反应不存在或难以检测。因此，人们对HCV的细胞免疫了解甚少。在本研究中，我们使用表达HCV包膜糖蛋白E1的伪狂犬病病毒减毒株和纯化的重组E1来检测E1蛋白是否是HCV免疫猪的T细胞识别的靶抗原。我们未能将E1蛋白鉴定为HCV免疫动物的T细胞识别的主要靶抗原。然而，这些细胞在活的HCV抗原刺激下在体外增殖。对HCV的淋巴细胞增殖反应严格依赖时间和剂量，并且可以在活的而非紫外线灭活的HCV刺激下诱导产生。去除实验表明，淋巴细胞增殖依赖于CD2 + CD8bright +淋巴细胞的存在，但CD2 + CD4 +细胞也对淋巴细胞增殖反应有贡献。接种10(7) 50%组织培养感染剂量的布雷西亚2.1.1株的动物的初次淋巴细胞增殖反应比接种10(3) 50%组织培养感染剂量的塞迪佩斯特株的动物更强。一个显著的发现是用HCV毒株接种动物后非抗原特异性淋巴细胞增殖增加。这种免疫现象可能掩盖了对该病毒的特异性T细胞反应。
