Pengue G, Caputo A, Rossi C, Barbanti-Brodano G, Lania L
Department of Genetics, Molecular and General Biology, University of Naples, Federico II, Italy.
J Virol. 1995 Oct;69(10):6577-80. doi: 10.1128/JVI.69.10.6577-6580.1995.
The evolutionarily conserved protein domain, called the Krüppel-associated box (KRAB), present in the amino termini of a large number of Krüppel-type zinc finger proteins is a strong repressor domain. In order to develop novel strategies to control human immunodeficiency virus type 1 (HIV-1) gene expression, we constructed a series of expression vectors expressing the wild-type Tat or Tat transdominant negative mutants fused to the KRAB repressor domain. We found that the KRAB domain tethered to the transactivating response element is able to suppress both basal and Tat-mediated activity of HIV-1 long terminal repeat-driven gene expression. These results suggest that the KRAB repressor domain fused to the Tat transdominant negative mutants can be successfully employed to control HIV-1 gene expression.
一种进化上保守的蛋白质结构域，称为克勒ppel相关盒（KRAB），存在于大量克勒ppel型锌指蛋白的氨基末端，是一个强大的抑制结构域。为了开发控制人类免疫缺陷病毒1型（HIV-1）基因表达的新策略，我们构建了一系列表达载体，这些载体表达与KRAB抑制结构域融合的野生型Tat或Tat反式显性负突变体。我们发现，与反式激活应答元件相连的KRAB结构域能够抑制HIV-1长末端重复序列驱动的基因表达的基础活性和Tat介导的活性。这些结果表明，与Tat反式显性负突变体融合的KRAB抑制结构域可成功用于控制HIV-1基因表达。
