Gennart J P, Baleux C, Verellen-Dumoulin C, Buchet J P, De Meyer R, Lauwerys R
Industrial Toxicology and Occupational Medicine Unit, Catholic University of Louvain, Brussels, Belgium.
Mutat Res. 1993 Mar;299(1):55-61. doi: 10.1016/0165-1218(93)90119-x.
Sister-chromatid exchange (SCE) in blood lymphocytes, serum tumors markers, carcinoembryonic antigen (CEA) and tissue polypeptide antigen (TPA), and urinary excretion of chromium, cobalt and nickel were determined in 26 male workers occupationally exposed to chromium, cobalt and nickel dust and in 25 controls matched for age and smoking habits. The differences in the urinary excretion of metals between exposed persons and controls were statistically significant. An analysis of variance on the SCE rank values revealed that both exposure status (exposed persons vs. controls) and smoking habits (smokers and former smokers vs. never smokers) had a statistically significant effect. For the tumor markers, the analysis of variance did not reveal a statistically significant difference between exposed persons and controls. However, CEA serum levels were significantly correlated not only with smoking habits but also with duration of exposure. As cobalt is only weakly mutagenic, these results suggest that the small amount of absorbed chromium and nickel may have been sufficient to induce SCE. The hypothesis that tumor markers may be increased in groups of subjects exposed to genotoxic substances deserves further study.
对26名职业性接触铬、钴和镍粉尘的男性工人以及25名年龄和吸烟习惯相匹配的对照者，测定了其血淋巴细胞中的姐妹染色单体交换（SCE）、血清肿瘤标志物癌胚抗原（CEA）和组织多肽抗原（TPA），以及尿中铬、钴和镍的排泄量。接触者与对照者尿中金属排泄量的差异具有统计学意义。对SCE秩次值进行方差分析显示，暴露状态（接触者与对照者）和吸烟习惯（吸烟者和既往吸烟者与从不吸烟者）均有统计学意义的影响。对于肿瘤标志物，方差分析未显示接触者与对照者之间有统计学意义的差异。然而，CEA血清水平不仅与吸烟习惯显著相关，还与接触时间显著相关。由于钴的致突变性较弱，这些结果表明，少量吸收的铬和镍可能足以诱导SCE。接触遗传毒性物质的人群中肿瘤标志物可能升高这一假说值得进一步研究。
