Blennow K, Wallin A, Gottfries C G, Månsson J E, Svennerholm L
Department of Psychiatry and Neurochemistry, University of Göteborg, Mölndal Hospital, Sweden.
J Neural Transm Park Dis Dement Sect. 1993;5(1):5-15. doi: 10.1007/BF02260910.
Concentration gradients in lumbar cerebrospinal fluid (CSF) for the monoamine metabolites homovanillic acid (HVA), 5-hydroxy-indoleacetic acid (5-HIAA) and 4-hydroxy-3-methoxyphenylglycol (HMPG) were studied in 9 healthy controls and 47 neuropsychiatric patients without diseases causing disturbed CSF circulation. In a serial sampling of the first 24 ml of CSF, steep concentration gradients between the first (0-4th ml) and last (21st-24th ml) portions of CSF were found for HVA (99 +/- 59% increase; p < 0.001) and 5-HIAA (88 +/- 54% increase; p < 0.001), while the concentration gradient was slight for HMPG (11 +/- 7% increase; p < 0.001). The existence of marked concentration gradients for the monoamine metabolites HVA and 5-HIAA gives further evidence for an active transport system for these metabolites and indicates that the lumbar CSF-HVA and 5-HIAA levels reflect the dopamine and serotonin metabolism in the brain. Moreover, the existence of pronounced concentration gradients for HVA and 5-HIAA stresses the importance of making analyses on a standardized volume of CSF.
在9名健康对照者和47名无脑脊液循环障碍疾病的神经精神疾病患者中，研究了单胺代谢产物高香草酸（HVA）、5-羟吲哚乙酸（5-HIAA）和4-羟基-3-甲氧基苯乙二醇（HMPG）在腰段脑脊液（CSF）中的浓度梯度。在前24毫升脑脊液的连续采样中，发现HVA（增加99±59%；p<0.001）和5-HIAA（增加88±54%；p<0.001）在脑脊液的第一部分（0-4毫升）和最后一部分（21-24毫升）之间存在陡峭的浓度梯度，而HMPG的浓度梯度较小（增加11±7%；p<0.001）。单胺代谢产物HVA和5-HIAA存在明显的浓度梯度，这进一步证明了这些代谢产物存在主动转运系统，并表明腰段脑脊液HVA和5-HIAA水平反映了大脑中的多巴胺和5-羟色胺代谢。此外，HVA和5-HIAA存在明显的浓度梯度，强调了对标准化体积脑脊液进行分析的重要性。
