Svärd S G, Kirsebom L A
Department of Microbiology, Biomedical Center, Uppsala, Sweden.
Nucleic Acids Res. 1993 Feb 11;21(3):427-34. doi: 10.1093/nar/21.3.427.
The location of the Escherichia coli RNase P cleavage site was studied both in vitro and in vivo. We show that selection of the cleavage site is dependent on the nucleotide at the cleavage site and the length of the acceptor-stem. Within the acceptor-stem the number of nucleotides on the 5'-half of the acceptor-stem appears to be the important determinant, rather than the number of base pairs in the acceptor-stem. We also demonstrate that the length of the T-stem and a G to C substitution at position 57 in the tRNA(Tyr)Su3 precursor influence the location of the cleavage site under certain conditions. With respect to the function of the subunits of RNase P our data suggest that the nucleotide at position 333 in M1 RNA, and the C5 protein, are important for the identification of the cleavage site.
我们在体外和体内研究了大肠杆菌核糖核酸酶P切割位点的位置。我们发现，切割位点的选择取决于切割位点处的核苷酸以及受体茎的长度。在受体茎内，受体茎5'-半部分的核苷酸数量似乎是重要的决定因素，而不是受体茎中的碱基对数量。我们还证明，在某些条件下，T茎的长度以及tRNA(Tyr)Su3前体中第57位的G到C替换会影响切割位点的位置。关于核糖核酸酶P亚基的功能，我们的数据表明，M1 RNA中第333位的核苷酸以及C5蛋白对于识别切割位点很重要。
