Sannita W G, Balestra V, DiBon G, Marotta V, Rosadini G
Department of Motor Sciences-Neurophysiopathology, University of Genoa, Italy.
Electroencephalogr Clin Neurophysiol. 1993 Apr;86(4):275-82. doi: 10.1016/0013-4694(93)90109-9.
Scopolamine in acute intramuscular doses of 0.25-0.75 mg reduced the P2-N3 flash-VEP amplitude and, in the quantitative EEG, the 8.5-12.0 Hz power and total power in 8 healthy young male volunteers. The effects on flash-VEP and EEG total power were dose dependent and were evident 30 min and 90 min respectively after drug administration, regardless of dose. The reduction in 8.5-12.0 Hz power was limited to the 0.50 and 0.75 mg doses. No systematic effects on the pattern-VEP were observed. Possible interferences with flash- or pattern-VEP amplitude of the scopolamine-induced EEG changes were identified and removed by regression analysis and computation of VEP residuals from the regression function. The P2-N3 flash-VEP residuals proved EEG independent and showed relationships with dose and time after drug administration that were superimposable on those of the original data, with comparable significance levels at the drug/placebo and pre/postdrug statistical comparisons. The results indicate that VEP estimates of drug effects which are independent from EEG changes can be identified in human studies and allow some inference on the cholinergic specificity of the systems affecting late flash-VEP components. The statistical approach used in this study is suitable for application in VEP studies when effects of interacting factors are to be expected.
急性肌肉注射剂量为0.25 - 0.75毫克的东莨菪碱，降低了8名健康年轻男性志愿者的P2 - N3闪光视觉诱发电位（VEP）波幅，并且在定量脑电图中，降低了8.5 - 12.0赫兹的功率和总功率。对闪光VEP和脑电图总功率的影响呈剂量依赖性，且无论剂量大小，给药后30分钟和90分钟时效应均明显。8.5 - 12.0赫兹功率的降低仅限于0.50毫克和0.75毫克剂量。未观察到对图形VEP有系统性影响。通过回归分析以及从回归函数计算VEP残差，识别并消除了东莨菪碱引起的脑电图变化对闪光或图形VEP波幅可能产生的干扰。P2 - N3闪光VEP残差被证明与脑电图无关，并且显示出与给药后的剂量和时间的关系，这些关系与原始数据的关系可叠加，在药物/安慰剂和给药前/给药后统计比较中具有相当的显著性水平。结果表明，在人体研究中可以识别出独立于脑电图变化的药物效应的VEP估计值，并能对影响晚期闪光VEP成分的系统的胆碱能特异性进行一些推断。本研究中使用的统计方法适用于预期存在相互作用因素影响的VEP研究。
