Segre M, Segre D
J Immunol. 1976 Mar;116(3):731-4.
The secondary response of young-adult and old BC3F1 mice to DNP was assessed by boosting spleen cells from primed mice with DNP-KLH, propagating them in diffusion chambers implanted into irradiated recipient mice, and by counting the DNP-specific PFC. There was no difference between young-adult and old cells in the antigen dose response and in the kinetics of the immune response. There were marked differences in the magnitude of the peak response. The response of 2-year-old mice was significantly smaller than the response of young-adult mice. Two and one half-year-old mice had the smallest response. In the old mice, there was an inverse correlation between the number of nucleated cells in the spleen and the log of the number of PFC.
通过用二硝基苯 - 钥孔戚血蓝蛋白（DNP-KLH）刺激致敏小鼠的脾细胞、将它们接种到植入受照射受体小鼠体内的扩散小室中培养，并计数二硝基苯特异性空斑形成细胞（PFC），评估成年和老年BC3F1小鼠对DNP的二次反应。成年和老年细胞在抗原剂量反应和免疫反应动力学方面没有差异。在峰值反应的幅度上存在显著差异。2岁小鼠的反应明显小于成年小鼠的反应。2.5岁小鼠的反应最小。在老年小鼠中，脾脏中有核细胞数量与PFC数量的对数之间存在负相关。
