Nyholm H C, Nielsen A L, Lyndrup J, Dreisler A, Thorpe S M
Department of Obstetrics and Gynecology, University of Copenhagen, Hvidovre Hospital, Denmark.
Int J Gynecol Pathol. 1993 Jul;12(3):246-52. doi: 10.1097/00004347-199307000-00008.
In 159 endometrial carcinomas, estrogen (ER) and progesterone receptors (PR) were determined biochemically by dextran-coated charcoal (DCC) assay and immunohistochemically (ICA) on frozen sections. ICA receptor content was estimated by a total histologic score (HSCORE), including all tissue components, and by a cancer HSCORE, including malignant cells only. These scores were closely correlated. A single biopsy was found to be representative for each tumor. ER-DCC status was positive in 90.3% and PR-DCC status in 92.2% of the tumors. ER total HSCORE was positive in 47% and PR total HSCORE in 89% of tumors. ER and PR correlated inversely with tumor grade (p < 0.001). Correlations were found between ER and PR content determined by either method (DCC: r = 0.77; ICA: r = 0.50), as well as between DCC and ICA content (ER: r = 0.52; PR: r = 0.76). The association between DCC and ICA was affected by the tumor grade: the DCC values decreased relatively more than total HSCOREs with increasing grade. The sensitivity of ICA against DCC assay was 56% for ER and 86% for PR. Maximal agreement between receptor status as determined by ICA and by DCC would result from a DCC cutoff level of 130 fmol/mg for ER and 114 fmol/mg for PR.
在159例子宫内膜癌中，通过葡聚糖包被活性炭（DCC）分析法和冷冻切片免疫组织化学法（ICA）对雌激素（ER）和孕激素受体（PR）进行了生化测定。ICA受体含量通过包括所有组织成分的总组织学评分（HSCORE）以及仅包括恶性细胞的癌HSCORE来估计。这些评分密切相关。发现单次活检对每个肿瘤具有代表性。90.3%的肿瘤ER-DCC状态为阳性，92.2%的肿瘤PR-DCC状态为阳性。47%的肿瘤ER总HSCORE为阳性，89%的肿瘤PR总HSCORE为阳性。ER和PR与肿瘤分级呈负相关（p<0.001）。两种方法（DCC：r=0.77；ICA：r=0.50）测定的ER和PR含量之间以及DCC和ICA含量之间（ER：r=0.52；PR：r=0.76）均存在相关性。DCC和ICA之间的关联受肿瘤分级影响：随着分级增加，DCC值下降相对比总HSCORE更多。ICA相对于DCC测定法对ER的敏感性为56%，对PR为86%。对于ER，DCC临界值为130 fmol/mg，对于PR为114 fmol/mg时，ICA和DCC确定的受体状态之间将达到最大一致性。
