没食子酸酯在过氧化氢诱导的细菌细胞毒性和SOS反应中的保护作用。

The protective role of gallic acid esters in bacterial cytotoxicity and SOS responses induced by hydrogen peroxide.

作者信息

Nakayama T, Hiramitsu M, Osawa T, Kawakishi S

机构信息

School of Food and Nutritional Sciences, University of Shizuoka, Japan.

出版信息

Mutat Res. 1993 Sep;303(1):29-34. doi: 10.1016/0165-7992(93)90005-g.

DOI:10.1016/0165-7992(93)90005-g

PMID:7690903

Abstract

The effects of gallic acid and its esters on H2O2-induced cytotoxicity, mutagenicity and SOS response were investigated in bacterial assay systems, i.e., the Ames test with Salmonella typhimurium TA104 and the SOS chromotest with E. coli PQ37. In the Ames test, gallic acid esters showed protective effects against H2O2-induced cytotoxicity and no effects on the number of revertant colonies. In the SOS chromotest, gallic acid esters lowered the SOS induction factor raised by H2O2. Throughout the study, the effects of gallic acid itself were weak or negligible, and lauryl gallate was most effective among the three gallic acid esters. This structure-activity relationship indicates the similarity of the protective effects of gallic acid esters on the H2O2-induced damages to both bacterial and mammalian cells.

摘要

在细菌检测系统中，即采用鼠伤寒沙门氏菌TA104进行的艾姆斯试验以及采用大肠杆菌PQ37进行的SOS显色试验，研究了没食子酸及其酯类对过氧化氢诱导的细胞毒性、致突变性和SOS反应的影响。在艾姆斯试验中，没食子酸酯对过氧化氢诱导的细胞毒性具有保护作用，且对回复突变菌落数无影响。在SOS显色试验中，没食子酸酯降低了由过氧化氢升高的SOS诱导因子。在整个研究中，没食子酸本身的作用较弱或可忽略不计，在三种没食子酸酯中，没食子酸月桂酯最为有效。这种构效关系表明，没食子酸酯对过氧化氢诱导的损伤对细菌和哺乳动物细胞的保护作用具有相似性。