一氧化氮合酶抑制剂对大鼠海马体长期增强效应的抑制作用具有温度和年龄依赖性。

The suppression of long-term potentiation in rat hippocampus by inhibitors of nitric oxide synthase is temperature and age dependent.

作者信息

Williams J H, Li Y G, Nayak A, Errington M L, Murphy K P, Bliss T V

机构信息

Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, England.

出版信息

Neuron. 1993 Nov;11(5):877-84. doi: 10.1016/0896-6273(93)90117-a.

DOI:10.1016/0896-6273(93)90117-a

PMID:7694601

Abstract

At room temperature (23 degrees C-25 degrees C), the induction of long-term potentiation (LTP) in area CA1 of slices from young male Sprague-Dawley rats was depressed by preincubation with the nitric oxide synthase inhibitors NG-nitro-L-arginine (L-NA, 100 microM) and NG-nitro-L-arginine methyl ester (L-NAME, 100 microM). The D isomers were ineffective under the same conditions. Hemoglobin (20 microM) reduced but did not completely block LTP. Neither L-NA (at concentrations up to 1 mM) nor hemoglobin (20 microM) had any significant effect on LTP in slices from adult rats at room temperature, or in young rats at 29 degrees C-30 degrees C. These results suggest that nitric oxide is unlikely to play a role in the induction of LTP under physiological conditions.

摘要

在室温（23摄氏度至25摄氏度）下，用一氧化氮合酶抑制剂NG-硝基-L-精氨酸（L-NA，100微摩尔）和NG-硝基-L-精氨酸甲酯（L-NAME，100微摩尔）预孵育，可抑制年轻雄性Sprague-Dawley大鼠脑片CA1区的长时程增强（LTP）诱导。在相同条件下，D-异构体无效。血红蛋白（20微摩尔）可降低但不能完全阻断LTP。在室温下，L-NA（浓度高达1毫摩尔）和血红蛋白（20微摩尔）对成年大鼠脑片或29摄氏度至30摄氏度下年轻大鼠脑片的LTP均无显著影响。这些结果表明，在生理条件下，一氧化氮不太可能在LTP的诱导中起作用。