Williams J H, Li Y G, Nayak A, Errington M L, Murphy K P, Bliss T V
Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, England.
Neuron. 1993 Nov;11(5):877-84. doi: 10.1016/0896-6273(93)90117-a.
At room temperature (23 degrees C-25 degrees C), the induction of long-term potentiation (LTP) in area CA1 of slices from young male Sprague-Dawley rats was depressed by preincubation with the nitric oxide synthase inhibitors NG-nitro-L-arginine (L-NA, 100 microM) and NG-nitro-L-arginine methyl ester (L-NAME, 100 microM). The D isomers were ineffective under the same conditions. Hemoglobin (20 microM) reduced but did not completely block LTP. Neither L-NA (at concentrations up to 1 mM) nor hemoglobin (20 microM) had any significant effect on LTP in slices from adult rats at room temperature, or in young rats at 29 degrees C-30 degrees C. These results suggest that nitric oxide is unlikely to play a role in the induction of LTP under physiological conditions.
在室温（23摄氏度至25摄氏度）下，用一氧化氮合酶抑制剂NG-硝基-L-精氨酸（L-NA，100微摩尔）和NG-硝基-L-精氨酸甲酯（L-NAME，100微摩尔）预孵育，可抑制年轻雄性Sprague-Dawley大鼠脑片CA1区的长时程增强（LTP）诱导。在相同条件下，D-异构体无效。血红蛋白（20微摩尔）可降低但不能完全阻断LTP。在室温下，L-NA（浓度高达1毫摩尔）和血红蛋白（20微摩尔）对成年大鼠脑片或29摄氏度至30摄氏度下年轻大鼠脑片的LTP均无显著影响。这些结果表明，在生理条件下，一氧化氮不太可能在LTP的诱导中起作用。
