Rudant E, Bourlioux P, Courvalin P, Lambert T
Centre d'Etudes Pharmaceutiques, Chatenay-Malabry, France.
FEMS Microbiol Lett. 1994 Nov 15;124(1):49-54. doi: 10.1016/0378-1097(94)90331-x.
The aac(6')-Ik gene which confers resistance to aminoglycosides in Acinetobacter sp. 6 CIP A165 was characterized. The resistance gene was identified as a coding sequence of 438 bases pairs corresponding to a protein with a calculated mass of 16627 Da. Alignment of aac(6')-Ik with aac(6')-Ig from Acinetobacter haemolyticus indicated 83% identity. Like aac(6')-Ig of A. haemolyticus and aac(6')-Ij of Acinetobacter sp. 13, the aac(6')-Ik gene was apparently located in the chromosome and was species specific. The high degree of identity between aac(6')-Ig and -Ik, compared with genomic DNA relationships between the host species, indicated that these genes have diverged from a common ancestor in a parental Acinetobacter species.
对不动杆菌属6 CIP A165中赋予对氨基糖苷类抗生素抗性的aac(6')-Ik基因进行了表征。该抗性基因被鉴定为一个438个碱基对的编码序列，对应于一个计算分子量为16627 Da的蛋白质。溶血不动杆菌的aac(6')-Ik与aac(6')-Ig比对显示83%的同一性。与溶血不动杆菌的aac(6')-Ig和不动杆菌属13的aac(6')-Ij一样，aac(6')-Ik基因显然位于染色体上且具有种特异性。与宿主物种之间的基因组DNA关系相比，aac(6')-Ig和-Ik之间的高度同一性表明这些基因在亲本不动杆菌物种中已从共同祖先分化而来。
