Olivier T V, Mitchell G M, Crowe D M, Romeo R, Gray K A, Morrison W A
Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria, Australia.
Br J Plast Surg. 1994 Dec;47(8):548-53. doi: 10.1016/0007-1226(94)90138-4.
A study was undertaken to evaluate the structure and patency of cold stored rabbit femoral veins following reinsertion as autografts for 3 weeks. The periods of cold ischaemic storage were 1, 2, 3 and 4 weeks (n = 10/gp) and 6 and 10 weeks (n = 6/gp). All rabbits were subject to 3 operations under general anaesthesia. In the first, a 4 cm segment of left femoral vein was harvested and stored at 4 degrees C for the specified ischaemic interval. Following storage the graft was microsurgically reinserted at a second operation into the right femoral artery of the donor rabbit. Three weeks later, graft patency was assessed by surgical exploration and the graft processed for light and electron microscopy. Patency rates remained over 80% after 3 weeks in all groups except the 10 week storage group where only 1 of 6 (17%) grafts was patent at 3 weeks. In all groups normal vein structure was absent, being replaced by thin walled necrotic areas or by neointimal ingrowth. The excellent patency rates achieved indicate it is possible to cold preserve extra lengths of vein grafts harvested at initial operation for reuse should regrafting be necessary.
进行了一项研究，以评估冷藏后的兔股静脉重新作为自体移植血管再植入3周后的结构和通畅情况。冷缺血储存时间分别为1周、2周、3周、4周（每组n = 10）以及6周和10周（每组n = 6）。所有兔子均在全身麻醉下接受3次手术。第一次手术时，切取4厘米长的左股静脉段，并在4℃下储存特定的缺血时间。储存后，在第二次手术中通过显微外科手术将移植血管重新植入供体兔的右股动脉。3周后，通过手术探查评估移植血管的通畅情况，并对移植血管进行光镜和电镜检查。除了10周储存组外，所有组在3周后的通畅率均保持在80%以上，在10周储存组中，6条移植血管中只有1条（17%）在3周时保持通畅。在所有组中，均未观察到正常的静脉结构，取而代之的是薄壁坏死区域或内膜增生。所取得的优异通畅率表明，在初次手术时采集的额外长度的静脉移植物，如果有必要再次移植，可以进行冷藏保存以备后用。
