Chen Y F, Tsai H Y, Wu T S
Department of Pharmacology, China Medical College, Taiwan.
Planta Med. 1995 Feb;61(1):2-8. doi: 10.1055/s-2006-957987.
In the present study, we extracted Angelica pubescens (AP) with various solvents in order to find the bioactive constituents that demonstrated analgesic and anti-inflammatory effects. The results were obtained as follows: (1) Methanol-, chloroform-, and ethyl acetate-extracts effectively reduced the pain that was induced by 1% acetic acid and a hot plate. (2) Methanol-, chloroform-, and ethyl acetate-extracts reduced the edema that was induced by 3% formalin or 1.5% carrageenan. (3) Sixteen compounds have been isolated and identified from the roots of AP. Among these compounds, columbianadin, columbianetin acetate, bergapten, umbelliferone, and caffeic acid significantly demonstrated anti-inflammatory and analgesic activities at 10 mg/kg. However, only osthole and xanthotoxin revealed anti-inflammatory activity. Isoimperatorin only demonstrated an analgesic effect. These results revealed that the anti-inflammatory and analgesic constituents from roots of AP were related to peripheral inhibition of inflammatory substances and to the influence on the central nervous system.
在本研究中，我们用各种溶剂提取独活（AP），以寻找具有镇痛和抗炎作用的生物活性成分。结果如下：（1）甲醇提取物、氯仿提取物和乙酸乙酯提取物能有效减轻1%乙酸和热板诱导的疼痛。（2）甲醇提取物、氯仿提取物和乙酸乙酯提取物能减轻3%福尔马林或1.5%角叉菜胶诱导的水肿。（3）已从独活根部分离并鉴定出16种化合物。在这些化合物中，蛇床子素、乙酸蛇床子素、佛手柑内酯、伞形花内酯和咖啡酸在10mg/kg时显著表现出抗炎和镇痛活性。然而，只有蛇床子素和花椒毒素显示出抗炎活性。异欧前胡素仅表现出镇痛作用。这些结果表明，独活根中的抗炎和镇痛成分与外周对炎症物质的抑制以及对中枢神经系统的影响有关。
