Severi M, Pauwels W, Van Hummelen P, Roosels D, Kirsch-Volders M, Veulemans H
Laboratory of Occupational Hygiene and Toxicology, Occupational and Insurance Medicine, Katholieke Universiteit Leuven, Belgium.
Scand J Work Environ Health. 1994 Dec;20(6):451-8. doi: 10.5271/sjweh.1375.
A field study was undertaken to investigate the effects of occupational styrene exposure on mandelic acid excretion and the formation of styrene-7,8-oxide hemoglobin adducts. Especially the sensitivity of a gas chromatography-mass spectrometry method for determining hemoglobin adducts was evaluated.
Over a four-week period, each individual of a group of 52 fiberglass-reinforced plastics workers was monitored once a week by the simultaneous measurement of styrene in the air and urinary postshift mandelic acid. In addition mandelic acid and hemoglobin adducts were monitored in a group of 24 unexposed referents. At the end of the monitoring period styrene-7,8-oxide adduct formation on N-terminal valine in hemoglobin was examined by gas chromatography-mass spectrometry according to the modified Edman degradation technique.
Personal air samples showed an average styrene exposure of 31 mg.m-3. The average postshift mandelic acid was 98 mg.g creatinine-1. For workers not wearing respirators and not showing breath ethanol, the correlation coefficient between styrene and mandelic acid was 0.78. The blood samples were analyzed for styrene-7,8-oxide adducts on hemoglobin. With a detection limit of 10 pmol.g-1, no styrene-7,8-oxide adducts were found under these exposure conditions.
Adduct formation in humans is less effective than in mice. In comparison with ethylene, styrene is at least 70 times less effective in forming hemoglobin adducts. Investigating adduct formation in humans at or below the exposure levels reported in this study would require a detection limit of about one order of magnitude better.
进行一项现场研究，以调查职业性苯乙烯暴露对扁桃酸排泄及苯乙烯 - 7,8 - 氧化物血红蛋白加合物形成的影响。特别评估了气相色谱 - 质谱法测定血红蛋白加合物的灵敏度。
在四周时间内，对一组52名玻璃纤维增强塑料工人，每周通过同时测量空气中的苯乙烯和下班后尿液中的扁桃酸进行一次监测。此外，对一组24名未暴露的对照者也监测了扁桃酸和血红蛋白加合物。在监测期结束时，根据改良的埃德曼降解技术，通过气相色谱 - 质谱法检测血红蛋白中N - 末端缬氨酸上苯乙烯 - 7,8 - 氧化物加合物的形成。
个人空气样本显示苯乙烯平均暴露量为31 mg·m⁻³。下班后扁桃酸的平均值为98 mg·g肌酐⁻¹。对于未佩戴呼吸器且未呼出乙醇的工人，苯乙烯与扁桃酸之间的相关系数为0.78。对血样进行血红蛋白上苯乙烯 - 7,8 - 氧化物加合物的分析。在这些暴露条件下，检测限为10 pmol·g⁻¹时，未发现苯乙烯 - 7,8 - 氧化物加合物。
人类体内加合物的形成比小鼠体内的效果差。与乙烯相比，苯乙烯形成血红蛋白加合物的效率至少低70倍。在本研究报告的暴露水平或以下调查人类体内加合物的形成，将需要检测限提高约一个数量级。
