Poe W J, Michael J G
Immunology. 1976 Feb;30(2):241-8.
An attempt was made to separate the antigenic and mitogenic properties of E. coli bacteria and bacterial lipopolysaccharide antigen inhibited the mitogenic response by the cultures but did not inhibit the induction of anti-LPS antibody or polyclonal antibody synthesis to SRBC. Dextran sulphate, acting as a B-cell mitogen, increased the mitogenic response in spleen cell cultures incubated with bacteria, but did not affect the production of anti-LPS antibody. Mild alkaline hydrolysis (0-1 N NaOH at 56 degrees) of LPS destroyed the mitogenic properties of the molecule, leaving the antigenic properties qualitatively intact. Harsher conditions of base hydrolysis destroyed both the mitogenic and antigenic properties of LPS, as determined by antigenicity in murine spleen cell cultures and haemagglutination inhibition tests.
曾尝试分离大肠杆菌的抗原性和促有丝分裂特性,细菌脂多糖抗原抑制了培养物的促有丝分裂反应,但未抑制抗脂多糖抗体的诱导或对绵羊红细胞的多克隆抗体合成。作为B细胞促有丝分裂原的硫酸葡聚糖增加了与细菌一起培养的脾细胞培养物中的促有丝分裂反应,但不影响抗脂多糖抗体的产生。脂多糖的温和碱水解(56℃下0-1N氢氧化钠)破坏了分子的促有丝分裂特性,使抗原性在质量上保持完整。通过小鼠脾细胞培养物中的抗原性和血凝抑制试验确定,更苛刻的碱水解条件破坏了脂多糖的促有丝分裂和抗原特性。