Tomoda M, Miyamoto H, Shimizu N, Gonda R, Ohara N
Kyoritsu College of Pharmacy, Tokyo, Japan.
Biol Pharm Bull. 1994 Nov;17(11):1456-9. doi: 10.1248/bpb.17.1456.
Two acidic polysaccharides, called rehmannan FS-I and rehmannan FS-II, were isolated from the raw root of Rehmannia glutinosa LIBOSCHITZ. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 5.8 x 10(4) and 6.6 x 10(4), respectively. Rehamannan FS-I is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid: D-glucuronic acid in the molar ratio of 68:40:4:84:3; rehmannan FS-II is composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid in the molar ratio of 18:15:4:33, in addition to small amounts of O-acetyl groups. About two-thirds (rehmannan FS-I) and about one half (rehamannan FS-II) of the hexuronic acid residues exist as methyl esters. Methylation analysis of the carboxyl-reduced derivatives and nuclear magnetic resonance studies indicated that their structural features include mainly both arabino-3,6-galactan and rhamno-galacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.
从地黄（Rehmannia glutinosa LIBOSCHITZ）的鲜根中分离出两种酸性多糖，分别称为地黄多糖FS-I和地黄多糖FS-II。它们在电泳和凝胶色谱上均表现为均一性，其分子量估计分别为5.8×10⁴和6.6×10⁴。地黄多糖FS-I由L-阿拉伯糖、D-半乳糖、L-鼠李糖、D-半乳糖醛酸、D-葡萄糖醛酸以68:40:4:84:3的摩尔比组成；地黄多糖FS-II由L-阿拉伯糖、D-半乳糖、L-鼠李糖、D-半乳糖醛酸以18:15:4:33的摩尔比组成，此外还含有少量的O-乙酰基。约三分之二（地黄多糖FS-I）和约一半（地黄多糖FS-II）的己糖醛酸残基以甲酯形式存在。对羧基还原衍生物的甲基化分析和核磁共振研究表明，它们的结构特征主要包括阿拉伯糖-3,6-半乳聚糖和鼠李糖-半乳糖醛酸聚糖类型的结构单元。在碳粒廓清试验中，这两种多糖均表现出显著的增强网状内皮系统活性。
