Ogawa K, Akagawa E, Nakamura K, Yamane K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):269-75. doi: 10.1099/13500872-141-2-269.
A 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome, located 306 kb downstream from the zero point of the physical map, was determined. Twenty-one putative ORFs were identified: two ORFs (Orf1 and Orf20) were consistent with the nucleotide and amino acid sequence of B. subtilis OrfJ, whose function is not known, and Pcp, respectively; four were found to display significant similarities to known proteins in data banks, i.e. 5-keto-3-deoxyglucarate dehydratase (Orf2), aldehyde dehydrogenase (Orf3) and two glucarate dehydratases (Orf4 and 5); three had considerable similarity to the sensor-regulator proteins of bacterial two-component signal transduction systems (Orf1, 11 and 12); two had considerably high levels of similarity to parts of known proteins (Orf13 and 19); and five showed low levels of similarity to known proteins in the data banks. The remaining six ORFs showed no similarity to known proteins.
测定了枯草芽孢杆菌染色体24°区域周围一段21548 bp的核苷酸序列，该区域位于物理图谱零点下游306 kb处。鉴定出21个推定的开放阅读框（ORF）：两个ORF（Orf1和Orf20）分别与功能未知的枯草芽孢杆菌OrfJ以及Pcp的核苷酸和氨基酸序列一致；4个与数据库中已知蛋白质具有显著相似性，即5-酮-3-脱氧葡萄糖酸脱水酶（Orf2）、醛脱氢酶（Orf3）以及两个葡萄糖二酸脱水酶（Orf4和Orf5）；3个与细菌双组分信号转导系统的传感调节蛋白具有相当的相似性（Orf1、11和12）；2个与已知蛋白质的部分区域具有相当高的相似性（Orf13和19）；5个与数据库中已知蛋白质具有低水平的相似性。其余6个ORF与已知蛋白质无相似性。
