Fujishima Y, Yamane K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Microbiology (Reading). 1995 Feb;141 ( Pt 2):277-9. doi: 10.1099/13500872-141-2-277.
The nucleotide sequence of approximately 10 kb at the 5' flanking region (32 degrees) of srfAA of the Bacillus subtilis chromosome was determined. Eleven putative ORFs were identified. Three of them (orf6, orf7 and orf8) coincided with known B. subtilis genes (comJ, comI and tlpC) encoding a competence-specific protein, a DNA-entry nuclease and a transducer-like protein, respectively. The products of two other ORFs showed similarity to GlnP of Escherichia coli (orf1) and beta-glucosidase A of B. polymyxa (orf5).
测定了枯草芽孢杆菌染色体srfAA基因5'侧翼区域（32°）约10 kb的核苷酸序列。鉴定出11个推定的开放阅读框（ORF）。其中3个（orf6、orf7和orf8）分别与枯草芽孢杆菌已知基因（comJ、comI和tlpC）一致，这些基因分别编码一种感受态特异性蛋白、一种DNA进入核酸酶和一种类转导蛋白。另外两个ORF的产物分别与大肠杆菌的GlnP（orf1）和多粘芽孢杆菌的β-葡萄糖苷酶A（orf5）相似。
