Campbell P, Ma S, Yeom B, McKellop H, Schmalzried T P, Amstutz H C
Implant Retrieval Laboratory, Los Angeles Orthopaedic Hospital, California 90007.
J Biomed Mater Res. 1995 Jan;29(1):127-31. doi: 10.1002/jbm.820290118.
A method of tissue digestion using sodium hydroxide was applied to the isolation and recovery of ultra-high-molecular-weight polyethylene (UHMWPE) particles from tissues around failed total hip replacements. Density gradient ultracentrifugation of the digested tissues was performed to separate the UHMWPE from cell debris and other particulates. Fourier transform infrared spectroscopy and differential scanning calorimetry (DSC) verified that the recovered particles were UHMWPE. When viewed by scanning electron microscopy, individual particles were clearly observed and were either rounded or elongated. The majority were submicron in size. The application of this method to the study of particles from periprosthetic tissues may elucidate aspects of biomaterial particle size and shape that are important to the biologic response to, and clinical outcome of, total joint replacement.
一种使用氢氧化钠进行组织消化的方法被应用于从失败的全髋关节置换周围组织中分离和回收超高分子量聚乙烯（UHMWPE）颗粒。对消化后的组织进行密度梯度超速离心，以将UHMWPE与细胞碎片和其他颗粒分离。傅里叶变换红外光谱和差示扫描量热法（DSC）证实回收的颗粒是UHMWPE。通过扫描电子显微镜观察时，可以清楚地观察到单个颗粒，它们要么是圆形的，要么是细长的。大多数颗粒尺寸在亚微米级别。这种方法应用于假体周围组织颗粒的研究，可能会阐明生物材料颗粒大小和形状的一些方面，这些方面对于全关节置换的生物学反应和临床结果很重要。
