Chen S, Slavin J, Fairley C K, Tabrizi S N, Borg A J, Billson V, Garland S M
Department of Microbiology and Pathology, Royal Women's Hospital, Carlton, Victoria 3053, Australia.
Genitourin Med. 1993 Aug;69(4):270-2. doi: 10.1136/sti.69.4.270.
To identify the prevalence of HPV DNA using the polymerase chain reaction (PCR) in neonatal foreskin and cervical specimens obtained at necropsy.
Foreskin and cervical specimens were obtained from consecutive neonates who had autopsies performed at The Royal Women's Hospital, Melbourne, from June 1991 to February 1992. Specimens were analysed for HPV DNA using the polymerase chain reaction and the L1 consensus primers and generic probes.
Specimens were obtained from 98 neonates, 52 males and 46 female. The mean gestational age of the neonates was 29 weeks (range 20-42). Eighty neonates died in utero, three during labour and 15 following delivery. Ninety four were delivered vaginally whilst four were delivered by caesarean section. Samples were collected a mean of 20 hours (range 2-48) from the time of delivery. In 30 cases there was evidence of autolytic change while in the remaining cases, the histology was well preserved. No evidence of HPV DNA was found in any of the samples using the L1 general primers (95% confidence interval 0-3.6%). Recent cervical cytology was available on 70 of the infant's mothers. Six had cytological evidence of HPV infection while the remainder were normal.
HPV DNA is uncommonly detected (by PCR) in foreskin and cervical specimens obtained from neonates.
运用聚合酶链反应（PCR）鉴定新生儿尸检时获取的包皮和宫颈标本中HPV DNA的流行情况。
包皮和宫颈标本取自1991年6月至1992年2月在墨尔本皇家妇女医院进行尸检的连续新生儿。使用聚合酶链反应以及L1共有引物和通用探针分析标本中的HPV DNA。
标本取自98例新生儿，其中52例为男性，46例为女性。新生儿的平均胎龄为29周（范围20 - 42周）。80例新生儿死于宫内，3例在分娩过程中死亡，15例在分娩后死亡。94例经阴道分娩，4例经剖宫产分娩。样本在分娩后平均20小时（范围2 - 48小时）采集。30例有自溶改变的证据，其余病例组织学保存良好。使用L1通用引物在任何样本中均未发现HPV DNA的证据（95%置信区间0 - 3.6%）。70例婴儿母亲近期有宫颈细胞学检查结果。6例有HPV感染的细胞学证据，其余均正常。
从新生儿获取的包皮和宫颈标本中很少检测到（通过PCR）HPV DNA。
