细菌鞭毛马达转速的波动分析
Fluctuation analysis of rotational speeds of the bacterial flagellar motor.
作者信息
Samuel A D, Berg H C
机构信息
Rowland Institute for Science, Cambridge, MA 02142, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3502-6. doi: 10.1073/pnas.92.8.3502.
Abstract
We measured the dependence of the variance in the rotation rate of tethered cells of Escherichia coli on the mean rotation rate over a regime in which the motor generates constant torque. This dependence was compared with that of broken motors. In either case, motor torque was augmented with externally applied torque. We show that, in contrast to broken motors, functioning motors in this regime do not freely rotationally diffuse and that the variance measurements are consistent with the predicted values of a stepping mechanism with exponentially distributed waiting times (a Poisson stepper) that steps approximately 400 times per revolution.
摘要
我们测量了在大肠杆菌系留细胞旋转速率的方差对平均旋转速率的依赖性，该过程处于电机产生恒定扭矩的状态。将这种依赖性与损坏的电机的依赖性进行了比较。在任何一种情况下，电机扭矩都通过外部施加的扭矩来增强。我们表明，与损坏的电机不同，处于这种状态下的正常运行电机不会自由地进行旋转扩散，并且方差测量结果与具有指数分布等待时间的步进机制（泊松步进器）的预测值一致，该步进器每转大约步进400次。
相似文献
1
Fluctuation analysis of rotational speeds of the bacterial flagellar motor.细菌鞭毛马达转速的波动分析
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3502-6. doi: 10.1073/pnas.92.8.3502.
2
Mechanical limits of bacterial flagellar motors probed by electrorotation.通过电旋转探测细菌鞭毛马达的机械极限
Biophys J. 1995 Jul;69(1):280-6. doi: 10.1016/S0006-3495(95)79900-3.
3
Study of the torque of the bacterial flagellar motor using a rotating electric field.利用旋转电场研究细菌鞭毛马达的扭矩
Biophys J. 1993 Mar;64(3):925-33. doi: 10.1016/S0006-3495(93)81454-1.
4
Torque generated by the flagellar motor of Escherichia coli.大肠杆菌鞭毛马达产生的扭矩。
Biophys J. 1993 Nov;65(5):2201-16. doi: 10.1016/S0006-3495(93)81278-5.
5
Torque generation by the flagellar rotary motor.鞭毛旋转马达产生的扭矩。
Biophys J. 1995 Apr;68(4 Suppl):163S-166S; discussion 166S-167S.
6
Torque-generating units of the bacterial flagellar motor step independently.细菌鞭毛马达的扭矩产生单元独立地进行步移。
Biophys J. 1996 Aug;71(2):918-23. doi: 10.1016/S0006-3495(96)79295-0.
7
Torque generated by the flagellar motor of Escherichia coli while driven backward.大肠杆菌鞭毛马达在反向驱动时产生的扭矩。
Biophys J. 1999 Jan;76(1 Pt 1):580-7. doi: 10.1016/S0006-3495(99)77226-7.
8
Torque generated by the bacterial flagellar motor close to stall.细菌鞭毛马达接近失速时产生的扭矩。
Biophys J. 1996 Dec;71(6):3501-10. doi: 10.1016/S0006-3495(96)79545-0.
9
A kinetic and stochastic analysis of crossbridge-type stepping mechanisms in rotary molecular motors.旋转分子马达中横桥型步进机制的动力学和随机分析。
Biophys J. 2005 Sep;89(3):1650-6. doi: 10.1529/biophysj.105.060095. Epub 2005 Jul 8.
10
On torque and tumbling in swimming Escherichia coli.关于游泳大肠杆菌中的扭矩和翻滚
J Bacteriol. 2007 Mar;189(5):1756-64. doi: 10.1128/JB.01501-06. Epub 2006 Dec 22.
引用本文的文献
1
Ligand sensing enhances bacterial flagellar motor output via stator recruitment.配体感应通过定子招募增强细菌鞭毛马达输出。
Elife. 2021 Apr 6;10:e62848. doi: 10.7554/eLife.62848.
2
More than Rotating Flagella: Lipopolysaccharide as a Secondary Receptor for Flagellotropic Phage 7-7-1.不止旋转菌毛：脂多糖作为菌毛噬菌体 7-7-1 的次级受体。
J Bacteriol. 2018 Sep 10;200(19). doi: 10.1128/JB.00363-18. Print 2018 Oct 1.
3
Organelles - understanding noise and heterogeneity in cell biology at an intermediate scale.细胞器——在中等尺度上理解细胞生物学中的噪声和异质性。
J Cell Sci. 2017 Mar 1;130(5):819-826. doi: 10.1242/jcs.181024. Epub 2017 Feb 9.
4
The Limiting Speed of the Bacterial Flagellar Motor.细菌鞭毛马达的极限速度
Biophys J. 2016 Aug 9;111(3):557-564. doi: 10.1016/j.bpj.2016.07.003.
5
Effect of the MotB(D33N) mutation on stator assembly and rotation of the proton-driven bacterial flagellar motor.MotB（D33N）突变对质子驱动的细菌鞭毛马达定子组装和旋转的影响。
Biophysics (Nagoya-shi). 2014 Jun 14;10:35-41. doi: 10.2142/biophysics.10.35. eCollection 2014.
6
Mannose-Binding Lectin Inhibits the Motility of Pathogenic Salmonella by Affecting the Driving Forces of Motility and the Chemotactic Response.甘露糖结合凝集素通过影响运动驱动力和趋化反应来抑制致病性沙门氏菌的运动。
PLoS One. 2016 Apr 22;11(4):e0154165. doi: 10.1371/journal.pone.0154165. eCollection 2016.
7
Coupling between switching regulation and torque generation in bacterial flagellar motor.细菌鞭毛马达中开关调节与转矩产生之间的耦合。
Phys Rev Lett. 2012 Apr 27;108(17):178105. doi: 10.1103/PhysRevLett.108.178105. Epub 2012 Apr 24.
8
Bacterial chemotaxis in an optical trap.细菌在光阱中的趋化性。
PLoS One. 2011 Apr 8;6(4):e18231. doi: 10.1371/journal.pone.0018231.
9
Modeling torque versus speed, shot noise, and rotational diffusion of the bacterial flagellar motor.建立细菌鞭毛马达的扭矩与速度、散粒噪声和旋转扩散的模型。
Phys Rev Lett. 2009 Dec 11;103(24):248102. doi: 10.1103/PhysRevLett.103.248102. Epub 2009 Dec 8.
10
Steps in the bacterial flagellar motor.细菌鞭毛马达的步骤。
PLoS Comput Biol. 2009 Oct;5(10):e1000540. doi: 10.1371/journal.pcbi.1000540. Epub 2009 Oct 23.
本文引用的文献
1
Torque generated by the flagellar motor of Escherichia coli.大肠杆菌鞭毛马达产生的扭矩。
Biophys J. 1993 Nov;65(5):2201-16. doi: 10.1016/S0006-3495(93)81278-5.
2
3
Fluctuation analysis of motor protein movement and single enzyme kinetics.运动蛋白运动的涨落分析与单酶动力学
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11782-6. doi: 10.1073/pnas.91.25.11782.
4
Successive incorporation of force-generating units in the bacterial rotary motor.细菌旋转马达中力产生单元的连续整合。
Nature. 1984;309(5967):470-2. doi: 10.1038/309470a0.
5
Dynamic properties of bacterial flagellar motors.细菌鞭毛马达的动态特性。
Nature. 1974 May 3;249(452):77-9. doi: 10.1038/249077a0.
6
Flagellar rotation and the mechanism of bacterial motility.鞭毛旋转与细菌运动机制
Nature. 1974 May 3;249(452):73-4. doi: 10.1038/249073a0.
7
Bacteria swim by rotating their flagellar filaments.细菌通过旋转其鞭毛丝来游动。
Nature. 1973 Oct 19;245(5425):380-2. doi: 10.1038/245380a0.
8
Constraints on flagellar rotation.鞭毛旋转的限制因素。
J Mol Biol. 1985 Aug 20;184(4):645-56. doi: 10.1016/0022-2836(85)90310-9.
9
The proton flux through the bacterial flagellar motor.质子通过细菌鞭毛马达的通量。
Cell. 1987 Jun 5;49(5):643-50. doi: 10.1016/0092-8674(87)90540-x.
10
The proton motive force in bacteria: a critical assessment of methods.细菌中的质子动力势：方法的批判性评估
Annu Rev Microbiol. 1985;39:219-42. doi: 10.1146/annurev.mi.39.100185.001251.
Zotero 插件