在常见大肠杆菌宿主背景中表达的β-内酰胺酶检测板的开发，用于评估新型β-内酰胺类抗生素。
Development of test panel of beta-lactamases expressed in a common Escherichia coli host background for evaluation of new beta-lactam antibiotics.
作者信息
Bradford P A, Sanders C C
机构信息
Department of Medical Microbiology, Creighton University, Omaha, Nebraska 68178, USA.
出版信息
Antimicrob Agents Chemother. 1995 Feb;39(2):308-13. doi: 10.1128/AAC.39.2.308.
Abstract
A test panel of 35 different beta-lactamases expressed in a common Escherichia coli host was created to compare the effect that each beta-lactamase had on susceptibility to various beta-lactam antibiotics. A comparison of the MICs obtained with this panel generally reflected differences in the substrate profiles of the various beta-lactamases examined. In addition, several strains of the panel were subjected to selection with porin-specific bacteriophages to obtain mutants lacking either the OmpC or OmpF porin protein. A mutation in either OmpC or OmpF did change the susceptibilities of certain strains expressing beta-lactamase to certain beta-lactam antibiotics. However, the loss of a single porin did not predictably alter susceptibility to any given beta-lactam drug. This panel of strains producing various beta-lactamases was found to be a useful tool for comparing the effects of different beta-lactamases and outer membrane permeability upon susceptibility to beta-lactam drugs.
摘要
构建了一个在常见大肠杆菌宿主中表达35种不同β-内酰胺酶的测试组，以比较每种β-内酰胺酶对各种β-内酰胺抗生素敏感性的影响。用该测试组获得的最低抑菌浓度（MIC）比较结果通常反映了所检测的各种β-内酰胺酶底物谱的差异。此外，测试组中的几株菌株用孔蛋白特异性噬菌体进行筛选，以获得缺失OmpC或OmpF孔蛋白的突变体。OmpC或OmpF的突变确实改变了某些表达β-内酰胺酶的菌株对某些β-内酰胺抗生素的敏感性。然而，单一孔蛋白的缺失并不能可预测地改变对任何给定β-内酰胺药物的敏感性。发现该产生各种β-内酰胺酶的菌株测试组是比较不同β-内酰胺酶和外膜通透性对β-内酰胺药物敏感性影响的有用工具。
相似文献
1
Development of test panel of beta-lactamases expressed in a common Escherichia coli host background for evaluation of new beta-lactam antibiotics.在常见大肠杆菌宿主背景中表达的β-内酰胺酶检测板的开发，用于评估新型β-内酰胺类抗生素。
Antimicrob Agents Chemother. 1995 Feb;39(2):308-13. doi: 10.1128/AAC.39.2.308.
2
The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics.外膜囊泡中孔蛋白和β-内酰胺酶在β-内酰胺类抗生素水解中的重要性。
Int J Mol Sci. 2020 Apr 17;21(8):2822. doi: 10.3390/ijms21082822.
3
Activities of beta-lactam antibiotics against Escherichia coli strains producing extended-spectrum beta-lactamases.β-内酰胺类抗生素对产超广谱β-内酰胺酶大肠杆菌菌株的活性。
Antimicrob Agents Chemother. 1990 May;34(5):858-62. doi: 10.1128/AAC.34.5.858.
4
Effects of beta-lactamases and omp mutation on susceptibility to beta-lactam antibiotics in Escherichia coli.β-内酰胺酶和外膜蛋白突变对大肠杆菌β-内酰胺类抗生素敏感性的影响。
Antimicrob Agents Chemother. 1989 Mar;33(3):382-6. doi: 10.1128/AAC.33.3.382.
5
Role of beta-lactamases and outer membrane proteins in multiple beta-lactam resistance of Enterobacter cloacae.β-内酰胺酶和外膜蛋白在阴沟肠杆菌多重β-内酰胺耐药中的作用
Antimicrob Agents Chemother. 1985 Apr;27(4):455-9. doi: 10.1128/AAC.27.4.455.
6
Frequent emergence of porin-deficient subpopulations with reduced carbapenem susceptibility in ESBL-producing Escherichia coli during exposure to ertapenem in an in vitro pharmacokinetic model.在体外药代动力学模型中，产 ESBL 大肠埃希菌在使用厄他培南时频繁出现孔蛋白缺陷亚群，这些亚群对碳青霉烯类药物的敏感性降低。
J Antimicrob Chemother. 2013 Jun;68(6):1319-26. doi: 10.1093/jac/dkt044. Epub 2013 Mar 10.
7
Factors determining resistance to beta-lactam combined with beta-lactamase inhibitors in Escherichia coli.决定大肠杆菌对β-内酰胺类与β-内酰胺酶抑制剂联合用药耐药性的因素。
J Antimicrob Chemother. 1991 May;27(5):569-75. doi: 10.1093/jac/27.5.569.
8
Selection and characterization of beta-lactam-resistant Escherichia coli K-12 mutants.耐β-内酰胺类大肠杆菌K-12突变体的筛选与鉴定
Antimicrob Agents Chemother. 1983 Apr;23(4):622-5. doi: 10.1128/AAC.23.4.622.
9
beta-Lactamases and beta-lactam resistance in Escherichia coli.大肠杆菌中的β-内酰胺酶与β-内酰胺抗性
Antimicrob Agents Chemother. 1985 Nov;28(5):703-5. doi: 10.1128/AAC.28.5.703.
10
Role of porin proteins OmpF and OmpC in the permeation of beta-lactams.孔蛋白OmpF和OmpC在β-内酰胺通透性中的作用。
Antimicrob Agents Chemother. 1982 Dec;22(6):942-8. doi: 10.1128/AAC.22.6.942.
引用本文的文献
1
Antibiotic susceptibility patterns of pathogens isolated from laboratory specimens at Livingstone Central Hospital in Zambia.赞比亚利文斯通中心医院实验室标本中分离出的病原体的抗生素敏感性模式。
PLOS Glob Public Health. 2022 Sep 14;2(9):e0000623. doi: 10.1371/journal.pgph.0000623. eCollection 2022.
2
Analysis and comparative genomics of R997, the first SXT/R391 integrative and conjugative element (ICE) of the Indian Sub-Continent.分析并比较 R997，即印度次大陆首个 SXT/R391 整合与共轭元件（ICE）。
Sci Rep. 2017 Aug 17;7(1):8562. doi: 10.1038/s41598-017-08735-y.
3
Pyrosequencing using the single-nucleotide polymorphism protocol for rapid determination of TEM- and SHV-type extended-spectrum beta-lactamases in clinical isolates and identification of the novel beta-lactamase genes blaSHV-48, blaSHV-105, and blaTEM-155.使用单核苷酸多态性协议进行焦磷酸测序，以快速测定临床分离株中TEM型和SHV型超广谱β-内酰胺酶，并鉴定新型β-内酰胺酶基因blaSHV-48、blaSHV-105和blaTEM-155。
Antimicrob Agents Chemother. 2009 Mar;53(3):977-86. doi: 10.1128/AAC.01155-08. Epub 2008 Dec 15.
4
Establishment of in vitro susceptibility testing methodologies and comparative activities of piperacillin in combination with the penem {beta}-lactamase inhibitor BLI-489.哌拉西林与青霉烯类β-内酰胺酶抑制剂BLI-489联合应用的体外药敏试验方法的建立及比较活性
Antimicrob Agents Chemother. 2009 Feb;53(2):370-84. doi: 10.1128/AAC.01047-08. Epub 2008 Nov 10.
5
Cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum beta-lactamase-producing Enterobacteriaceae.头孢吡肟、哌拉西林-他唑巴坦与产超广谱β-内酰胺酶肠杆菌科细菌检测中的接种物效应
Antimicrob Agents Chemother. 2001 Dec;45(12):3548-54. doi: 10.1128/AAC.45.12.3548-3554.2001.
6
Real-time PCR and melting curve analysis for reliable and rapid detection of SHV extended-spectrum beta-lactamases.用于可靠快速检测超广谱β-内酰胺酶SHV的实时聚合酶链反应及熔解曲线分析
Antimicrob Agents Chemother. 2001 Jun;45(6):1730-6. doi: 10.1128/AAC.45.6.1730-1736.2001.
7
Ability of the VITEK 2 advanced expert system To identify beta-lactam phenotypes in isolates of Enterobacteriaceae and Pseudomonas aeruginosa.VITEK 2高级专家系统鉴定肠杆菌科细菌和铜绿假单胞菌分离株中β-内酰胺表型的能力。
J Clin Microbiol. 2000 Feb;38(2):570-4. doi: 10.1128/JCM.38.2.570-574.2000.
8
Use of an isogenic Escherichia coli panel to design tests for discrimination of beta-lactamase functional groups of Enterobacteriaceae.使用同基因大肠杆菌组设计用于区分肠杆菌科β-内酰胺酶功能组的检测方法。
Antimicrob Agents Chemother. 1999 Mar;43(3):630-3. doi: 10.1128/AAC.43.3.630.
9
Detection of extended-spectrum beta-lactamase (ESBL)-producing strains by the Etest ESBL screen.采用Etest ESBL筛选法检测产超广谱β-内酰胺酶（ESBL）菌株。
J Clin Microbiol. 1996 Aug;34(8):1880-4. doi: 10.1128/jcm.34.8.1880-1884.1996.
本文引用的文献
1
Cefepime: the next generation?头孢吡肟：新一代产品？
Clin Infect Dis. 1993 Sep;17(3):369-79.
2
Detection of PSE-2 beta-lactamase in enterobacteria.肠杆菌科细菌中PSE-2β-内酰胺酶的检测
Antimicrob Agents Chemother. 1984 Feb;25(2):268-72. doi: 10.1128/AAC.25.2.268.
3
Comparison of polyacrylamide and agarose gel thin-layer isoelectric focusing for the characterization of beta-lactamases.聚丙烯酰胺和琼脂糖凝胶薄层等电聚焦用于β-内酰胺酶表征的比较
Antimicrob Agents Chemother. 1983 Aug;24(2):186-9. doi: 10.1128/AAC.24.2.186.
4
The Antibacterial activity in vitro and beta-lactamase stability of the new cephalosporin HR 810 in comparison with five other cephalosporins and two aminoglycosides.新型头孢菌素HR 810与其他五种头孢菌素及两种氨基糖苷类药物相比的体外抗菌活性及β-内酰胺酶稳定性
Infection. 1983 Sep-Oct;11(5):275-9. doi: 10.1007/BF01641262.
5
Compatibility and molecular properties of plasmid Rms 149 in Pseudomonas aeruginosa and Escherichia coli.铜绿假单胞菌和大肠杆菌中质粒Rms 149的相容性及分子特性
Plasmid. 1980 Jan;3(1):1-6. doi: 10.1016/s0147-619x(80)90029-3.
6
Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein.大肠杆菌K-12中外膜蛋白合成的调控：ompC缺失影响OmpF蛋白的表达。
J Bacteriol. 1984 Aug;159(2):555-63. doi: 10.1128/jb.159.2.555-563.1984.
7
Penicillinase synthesis controlled by infectious R factors in Enterobacteriaceae.肠杆菌科中由传染性R因子控制的青霉素酶合成
Nature. 1965 Oct 16;208(5007):239-41. doi: 10.1038/208239a0.
8
The purification and properties of the -lactamase specified by the resistance factor R-1818 in Escherichia coli and Proteus mirabilis.大肠杆菌和奇异变形杆菌中由抗性因子R - 1818所指定的β - 内酰胺酶的纯化及特性
Biochem J. 1971 Jul;123(4):493-500. doi: 10.1042/bj1230493.
9
R-factor-mediated beta-lactamases that hydrolyze oxacillin: evidence for two distinct groups.R因子介导的可水解苯唑西林的β-内酰胺酶：两个不同组别的证据。
J Bacteriol. 1974 Aug;119(2):351-6. doi: 10.1128/jb.119.2.351-356.1974.
10
R factor-mediated and chromosomal resistance to ampicillin in Escherichia coli.大肠杆菌中R因子介导的及染色体介导的对氨苄青霉素的耐药性
Antimicrob Agents Chemother. 1974 Feb;5(2):186-91. doi: 10.1128/AAC.5.2.186.
Zotero 插件