Tsuji S, Kawano S, Tsujii M, Fusamoto H, Kamada T
First Department of Medicine, Osaka University School of Medicine, Suita, Japan.
Dig Dis Sci. 1995 May;40(5):1132-9. doi: 10.1007/BF02064211.
Hepatocyte growth factor (HGF) stimulates the growth of hepatocytes and other epithelial cells. A gene for the HGF receptor, c-met, is detected in the intestinal tract and the liver, as well as in gastric carcinoma cells. However, the role of HGF in the regeneration of the normal gastric mucosa is not known. The purpose of the present study was to elucidate the effects of HGF on the morphogenesis of cultured gastric mucosal cells and to evaluate the role of HGF and c-met in the healing process in rat gastric mucosa. The cultured gastric mucosal cells developed a branching morphology in a collagen matrix supplemented with HGF or fetal calf serum. They did not form this morphology on a plastic dish or in the collagen without HGF or the serum. In an in vivo study, total RNA was extracted from rat gastric mucosa 6, 24, 48, and 96 hr after the exposure to a solution of 0.6 M HCl. HGF messenger RNA was not detected, but c-met was expressed in the mucosa. The increased expression of c-met was followed by healing of the mucosal injury. These results indicate that HGF plays important roles in the morphogenesis of gastric mucosal cells and that the HGF receptor gene participates in the healing process of gastric mucosa.
肝细胞生长因子（HGF）可刺激肝细胞和其他上皮细胞的生长。在肠道、肝脏以及胃癌细胞中可检测到HGF受体c-met的基因。然而，HGF在正常胃黏膜再生中的作用尚不清楚。本研究的目的是阐明HGF对培养的胃黏膜细胞形态发生的影响，并评估HGF和c-met在大鼠胃黏膜愈合过程中的作用。在补充有HGF或胎牛血清的胶原基质中，培养的胃黏膜细胞呈现出分支形态。在塑料培养皿中或不含HGF或血清的胶原中，它们不会形成这种形态。在一项体内研究中，在大鼠胃黏膜暴露于0.6 M盐酸溶液后6、24、48和96小时提取总RNA。未检测到HGF信使RNA，但c-met在黏膜中表达。c-met表达增加后，黏膜损伤得以愈合。这些结果表明，HGF在胃黏膜细胞的形态发生中起重要作用，并且HGF受体基因参与胃黏膜的愈合过程。
