von Stedingk L V, Olsson I, Hanson H S, Asbrink E, Hovmark A
Department of Clinical Microbiology, Karolinska Hospital, Stockholm, Sweden.
Eur J Clin Microbiol Infect Dis. 1995 Jan;14(1):1-5. doi: 10.1007/BF02112610.
The aim of this study was to evaluate the polymerase chain reaction (PCR) as a diagnostic tool for Lyme borreliosis on large numbers of samples from clinically well-defined cases of early and late cutaneous borreliosis. Skin biopsy specimens from patients with erythema migrans and acrodermatitis chronica atrophicans were analysed blindly together with an equal number of control biopsies. Using two different dilutions of each DNA specimen increased the number of total positives detected. All of the 76 control biopsies were PCR negative. Biopsy specimens from 18 of 26 (69%) erythema migrans lesions and from 22 of 36 (61%) acrodermatitis chronica atrophicans lesions were PCR positive. Fourteen post-therapy biopsies from patients with acrodermatitis chronica atrophicans were all negative, supporting the opinion that antibiotic therapy is successful in this chronic manifestation of Lyme borreliosis.
本研究的目的是评估聚合酶链反应（PCR）作为一种诊断工具，用于检测大量来自临床明确诊断的早期和晚期皮肤型莱姆病病例的样本。对患有游走性红斑和慢性萎缩性肢端皮炎患者的皮肤活检标本进行了盲法分析，并与相同数量的对照活检标本一起分析。对每个DNA标本使用两种不同的稀释度增加了检测到的总阳性数量。所有76份对照活检标本的PCR结果均为阴性。26例游走性红斑病变中的18例（69%）以及36例慢性萎缩性肢端皮炎病变中的22例（61%）的活检标本PCR结果为阳性。14份来自慢性萎缩性肢端皮炎患者的治疗后活检标本均为阴性，支持了抗生素治疗对莱姆病这种慢性表现有效的观点。
