用于检测猪体内猪繁殖与呼吸综合征病毒抗体的阻断酶联免疫吸附测定法和免疫过氧化物酶单层测定法的比较研究
Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs.
作者信息
Houben S, Callebaut P, Pensaert M B
机构信息
Laboratory of Virology, Faculty of Veterinary Medicine, University of Gent, Merelbeke, Belgium.
出版信息
J Virol Methods. 1995 Jan;51(1):125-8. doi: 10.1016/0166-0934(94)00135-4.
Abstract
A blocking enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to the porcine reproductive and respiratory syndrome virus (PRRSV) in pig sera was developed and compared with the immunoperoxidase monolayer assay (IPMA), the most widely used serological diagnostic test in Europe. The blocking ELISA was specific and more sensitive than the IPMA when applied to field sera and to sera which were collected early after an experimental infection with PRRSV. Problems with high background activity as observed in IPMA or indirect ELISA were not encountered.
摘要
开发了一种用于检测猪血清中猪繁殖与呼吸综合征病毒（PRRSV）抗体的阻断酶联免疫吸附测定（ELISA），并将其与免疫过氧化物酶单层测定（IPMA）进行比较，IPMA是欧洲使用最广泛的血清学诊断试验。当应用于现场血清和PRRSV实验感染后早期采集的血清时，阻断ELISA具有特异性且比IPMA更敏感。未遇到在IPMA或间接ELISA中观察到的高背景活性问题。
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