Steube K G, Grunicke D, Drexler H G
DSM - German Collection of Microorganisms and Cell Cultures, Department of Human and Animal Cell Cultures, Braunschweig.
In Vitro Cell Dev Biol Anim. 1995 Feb;31(2):115-9. doi: 10.1007/BF02633971.
One of the major problems in cell culturing is the misidentification or cross-contamination of authentic continuous cell lines. We applied a rapid and efficient isoelectric focusing (IEF) technique for the routine analysis to detect interspecies contamination of cell cultures and for the identification of unknown animal cell lines. The method is based on the isoelectric separation of a specific set of intracellular enzymes which can be used to distinguish between cell lines of human, murine, or other mammalian origin. By means of preformed agarose gels, standardized conditions and equipment, this technique is especially applicable for routine work and allows the analysis of a large number of unknown samples with reproducible results. One hundred seventy-seven cell lines which have been sent to the Department of Human and Animal Cell Cultures at the DSM (Deutsche Sammlung von Mikroorganismen and Zellkulturen) were analyzed for species authentication; only three cell lines were found not to be of the presumed species. Our study strongly emphasizes standardized IEF as an efficient and rapid method for routinely monitoring the authenticity of cell lines.
细胞培养中的一个主要问题是正宗连续细胞系的错误识别或交叉污染。我们应用了一种快速高效的等电聚焦（IEF）技术进行常规分析，以检测细胞培养物中的种间污染并鉴定未知动物细胞系。该方法基于特定一组细胞内酶的等电分离，这些酶可用于区分人源、鼠源或其他哺乳动物来源的细胞系。借助预制琼脂糖凝胶、标准化条件和设备，该技术特别适用于常规工作，并能对大量未知样品进行分析，结果具有可重复性。对送往德国微生物和细胞培养物保藏中心（DSM）人类和动物细胞培养部的177个细胞系进行了物种鉴定分析；仅发现三个细胞系并非预期的物种。我们的研究强烈强调标准化等电聚焦是一种用于常规监测细胞系真实性的高效快速方法。
