Schlossberg D, Brooks J B, Shulman J
J Clin Microbiol. 1976 Mar;3(3):239-45. doi: 10.1128/jcm.3.3.239-245.1976.
Cerebrospinal fluid (CSF) from eight patients with cryptococcal meningitis, from ten patients with viral meningitis, and from four control patients without meningitis were analyzed by electron-capture gas-liquid chromatography (EC-GLC). All cryptococcal specimens had similar EC-GLC profiles, and these differed from those of the controls. Viral EC-GLC patterns were different from those obtained with specimens from the patients with cryptococcal infection and from the controls. In addition, specimens from patients with various types of viral infections gave profiles that differed from each other. Two normal CSFs were inoculated with Cryptococcus neoformans; aliquots of these cultures showed an EC-GLC pattern very similar to that seen in CSF of patients with cryptococcal meningitis. The EC-GLC procedure is rapid, reproducible, and easy to perform and may hold promise as an additional aid in the diagnosis of cryptococcal infection.
采用电子捕获气液色谱法（EC-GLC）对8例隐球菌性脑膜炎患者、10例病毒性脑膜炎患者以及4例无脑膜炎的对照患者的脑脊液（CSF）进行了分析。所有隐球菌标本具有相似的EC-GLC图谱，且这些图谱与对照者的图谱不同。病毒性EC-GLC模式与隐球菌感染患者标本及对照者标本所获得的模式不同。此外，各种类型病毒感染患者的标本给出的图谱彼此不同。将两例正常脑脊液接种新型隐球菌；这些培养物的等分试样显示出的EC-GLC模式与隐球菌性脑膜炎患者脑脊液中所见模式非常相似。EC-GLC方法快速、可重复且易于操作，可能有望作为隐球菌感染诊断的辅助手段。
