一种用于分散脾细胞培养物体外免疫的简化程序。
A simplified procedure for in vitro immunization of dispersed spleen cell cultures.
作者信息
Kamo I, Pan S H, Friedman H
出版信息
J Immunol Methods. 1976;11(1):55-62. doi: 10.1016/0022-1759(76)90018-1.
Dispersed spleen cell suspensions from normal mice were immunized with sheep erythrocytes in flat bottom vial tubes containing a relatively small amount of tissue culture medium fortified with a 'nutrient cocktail'. The magnitude of the number of antibody plaque forming cells appearing in such tube cultures was equivalent or greater than that obtained in Marbrook vessels in the Mishell--Dutton system. Omission of the nutrient cocktail on the day of culture initiation, or the use of round bottom vials, resulted in much lower PFC responses.
从正常小鼠中分离出的脾细胞悬液，在含有相对少量添加了“营养鸡尾酒”的组织培养基的平底小瓶中用绵羊红细胞进行免疫。在这种试管培养中出现的抗体斑块形成细胞数量的规模，与在米谢尔-达顿系统的马尔布鲁克容器中获得的相当或更大。在培养开始当天省略营养鸡尾酒，或使用圆底小瓶，会导致低得多的斑块形成细胞反应。
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