Misra R N, Debata N K, Nagend A, Hyder T S, Ohri V C
Reader, Department of Microbiology, Armed Forces Medical College, Pune-40.
Associate Professor, Department of Microbiology, Armed Forces Medical College, Pune-40.
Med J Armed Forces India. 1998 Jul;54(3):222-224. doi: 10.1016/S0377-1237(17)30548-8. Epub 2017 Jun 26.
Sixteen strains of were isolated from cases of diarrhoea. Out of these, 12 (75%) were identified as and 4 (25%) as Vibrio cholerae El Tor by standard biochemical and serological tests. Modified CAMP reaction in sheep blood agar showed that 0139 produced moderate hemolysis, El Tor produced wider zone of hemolysis whereas Classical produced no zone of hemolysis (CAMP negative). Break point minimum inhibitory concentration (MIC) by agar dilution method showed that all 0139 strains were resistant to ampicillin 8 mg/L, streptomycin 1 mg/L, chloramphenicol 8 mg/L, sulphamethoxazole 32 mg/L and trimethoprim 0.3-128 mg/L, 58.3% were sensitive to gentamicin 1 mg/L, and all were sensitive to norfloxacin 1 mg/L and cefotaxime 1 mg/L. Resistance to trimethoprim, sulphamethoxazole, ampicillin and gentamicin in 5 strains could be transferred to K-12 by conjugation experiment at a rate of 5×10 to 4×10. Distinct plasmid bands of 35.8 mega daltons could be seen in agarose gel electrophoresis.
从腹泻病例中分离出16株[未提及的菌株名称]。其中，通过标准生化和血清学检测，12株（75%）被鉴定为[未提及的菌株名称]，4株（25%）被鉴定为埃尔托型霍乱弧菌。在羊血琼脂平板上进行的改良CAMP反应显示，0139型产生中度溶血，埃尔托型产生较宽的溶血区，而古典生物型不产生溶血区（CAMP阴性）。采用琼脂稀释法测定的断点最低抑菌浓度（MIC）表明，所有0139型菌株对氨苄西林8 mg/L、链霉素1 mg/L、氯霉素8 mg/L、磺胺甲恶唑32 mg/L和甲氧苄啶0.3 - 128 mg/L耐药，58.3%的菌株对庆大霉素1 mg/L敏感，所有菌株对诺氟沙星1 mg/L和头孢噻肟1 mg/L敏感。通过接合试验，5株菌株对甲氧苄啶、磺胺甲恶唑、氨苄西林和庆大霉素的耐药性可按5×10至4×10的频率转移至大肠杆菌K - 12。在琼脂糖凝胶电泳中可看到35.8兆道尔顿的明显质粒条带。
