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本文引用的文献

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Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis.简化聚合酶链反应的进展及其在结核病诊断中的应用。
J Clin Microbiol. 1993 Apr;31(4):776-82. doi: 10.1128/jcm.31.4.776-782.1993.
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Simple colorimetric microtiter plate hybridization assay for detection of amplified Mycobacterium leprae DNA.用于检测扩增的麻风分枝杆菌DNA的简易比色微量滴定板杂交试验。
J Clin Microbiol. 1993 Mar;31(3):665-70. doi: 10.1128/jcm.31.3.665-670.1993.
3
Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples.用于检测临床样本中结核分枝杆菌的巢式聚合酶链反应
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Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.在常规分枝杆菌学实验室中大规模使用聚合酶链反应检测结核分枝杆菌。
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Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction.在临床实验室中通过聚合酶链反应直接检测呼吸道标本中的结核分枝杆菌。
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Detection and identification of Mycobacterium tuberculosis by DNA amplification.通过DNA扩增检测和鉴定结核分枝杆菌。
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用于检测痰液样本中扩增的结核分枝杆菌DNA的比色微孔板杂交测定法。

Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

作者信息

Cho S N, van der Vliet G M, Park S, Baik S H, Kim S K, Chong Y, Kolk A H, Klatser P R, Kim J D

机构信息

Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.

出版信息

J Clin Microbiol. 1995 Mar;33(3):752-4. doi: 10.1128/jcm.33.3.752-754.1995.

DOI:10.1128/jcm.33.3.752-754.1995
PMID:7751391
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228029/
Abstract

We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

摘要

我们开发了一种比色微孔板杂交检测法（CoMPHA），用于特异性检测5'-生物素化扩增的结核分枝杆菌DNA。CoMPHA的光密度与纯化模板DNA的初始量相对应。在此，我们表明CoMPHA有助于区分PCR阳性和PCR阴性样本。