用于检测痰液样本中扩增的结核分枝杆菌DNA的比色微孔板杂交测定法。
Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.
作者信息
Cho S N, van der Vliet G M, Park S, Baik S H, Kim S K, Chong Y, Kolk A H, Klatser P R, Kim J D
机构信息
Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
出版信息
J Clin Microbiol. 1995 Mar;33(3):752-4. doi: 10.1128/jcm.33.3.752-754.1995.
Abstract
We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.
摘要
我们开发了一种比色微孔板杂交检测法（CoMPHA），用于特异性检测5'-生物素化扩增的结核分枝杆菌DNA。CoMPHA的光密度与纯化模板DNA的初始量相对应。在此，我们表明CoMPHA有助于区分PCR阳性和PCR阴性样本。
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