Pizza F X, Mitchell J B, Davis B H, Starling R D, Holtz R W, Bigelow N
Department of Physical Education, Texas Christian University, Fort Worth 76129, USA.
Med Sci Sports Exerc. 1995 Mar;27(3):363-70.
The purpose of this study was to determine the effect of downhill and level running on circulating leukocyte and lymphocyte subsets and T lymphocyte activation. Using a random cross-over design, 10 runners completed two trials of 60 min of level running (0% grade; LR) and downhill running (-10% grade; DHR) at 70% of level VO2max. Blood samples were obtained preexercise and immediately postexercise (POST) and at 1.5, 12, 24, and 48 h of recovery. Creatine kinase activity peaked at 12 h of recovery from DHR and was not significantly altered following LR. The number of total T, CD16+, CD3+CD56+ cells were significantly higher POST DHR compared with LR. Leukocyte and neutrophil counts were significantly higher at 1.5 and 12 h of recovery from DHR compared with LR. The number of activated CD8+ cells (CD25+ CD8+) was significantly higher at 12 h of DHR compared to LR. Total T cells were significantly reduced at various time points during the 48 h of recovery from LR and DHR. In summary, DHR relative to LR resulted in a greater mobilization of lymphocytes (post), neutrophils (1.5-12 h of recovery) and activation of CD8+ cells at 12 h of recovery. In addition, reductions in circulating T lymphocyte subsets occurred following both conditions.
本研究的目的是确定下坡跑和平地跑对循环白细胞、淋巴细胞亚群及T淋巴细胞活化的影响。采用随机交叉设计，10名跑步者以平地最大摄氧量的70%完成了两次60分钟的试验，一次是平地跑（坡度0%；LR），另一次是下坡跑（坡度-10%；DHR）。在运动前、运动后即刻（POST）以及恢复1.5、12、24和48小时采集血样。肌酸激酶活性在DHR恢复12小时时达到峰值，LR后无显著变化。与LR相比，DHR后总T细胞、CD16+细胞、CD3+CD56+细胞数量显著更高。与LR相比，DHR恢复1.5和12小时时白细胞和中性粒细胞计数显著更高。与LR相比，DHR 12小时时活化CD8+细胞（CD25+CD8+）数量显著更高。LR和DHR恢复48小时期间的各个时间点，总T细胞均显著减少。总之，与LR相比，DHR导致淋巴细胞（运动后）、中性粒细胞（恢复1.5 - 12小时）动员增加，且恢复12小时时CD8+细胞活化增加。此外，两种情况下循环T淋巴细胞亚群均减少。
